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需要flgM来规避NLRC4介导的宿主保护对鞭状沙门氏菌

Américo Harry López-Yglesias¹, Chun-Chi Lu¹, Marvin A Lai¹

¹Department of Laboratory Medicine and Pathology, University of Washington , Seattle, Washington, USA.

Infection and immunity

|August 28, 2023

概括

通过NLRC4炎症体识别沙门氏菌鞭是对宿主防御粘膜感染的关键. 沙门氏菌逃避这种途径会增加其毒性和肠道损伤.

科学领域:

免疫学免疫学免疫学
微生物学微生物学
传染性疾病传染性疾病

背景情况:

沙门氏菌 (Salmonella enterica serovar Typhimurium) 是全球胃肠炎的重要原因,对弱势群体构成严重威胁.
NLRC4炎症体在对沙门氏菌的天生的免疫力中发挥着关键作用,在全身感染和粘膜感染中具有不同的功能.
免疫系统识别了旗素和非旗素分子,但主要的保护途径尚不清楚.

研究的目的:

调查假设NLRC4炎症酶识别沙门氏菌鞭毛素是感染期间的主要保护机制.
在系统性和粘膜感染模型中阐明鞭毛蛋白在调解NLRC4依赖宿主耐药性的作用.
了解FlgM蛋白在沙门氏菌逃避NLRC4炎症体中的作用及其对病毒毒性的影响.

主要方法:

在小鼠感染模型中利用了野生型,缺乏鞭毛素和产生鞭毛素过量的沙门氏菌Typhimurium菌株.
在系统性和粘膜感染阶段比较宿主耐药性.
评估了鞭蛋白表达水平和flgM基因删除对沙门氏菌感染和NLRC4炎症酶激活的影响.

主要成果:

沙门氏菌 Typhimurium 在感染的全身阶段有效地规避NLRC4介导的先天免疫力.
在粘膜沙门氏菌感染期间,NLRC4炎症体对旗素的识别是保护性天生的免疫的主要途径.
删除flgM导致构成性鞭毛素表达,显著限制系统和粘膜沙门氏菌感染以NLRC4炎症酶依赖的方式.

结论:

在粘膜感染期间NLRC4炎症体对沙门氏菌鞭素的识别是对这种肠道病原体的主要先天性保护途径.
沙门氏菌对NLRC4炎症体的flgM介导的逃避增强了它的毒性,并导致肠道组织的破坏.
准鞭毛素-NLRC4相互作用是对抗沙门氏菌感染的潜在策略.

关键词:

在NLRC4中,沙门氏菌是一种沙门氏菌.标签: 标志标志标志标志标志一些炎症一些炎症这是一种炎症炎症炎症炎症.这是先天免疫力.粘膜免疫力粘膜免疫力

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