tRNAqueuosine修饰参与了细菌中的生物膜形成和毒性
Jorge Díaz-Rullo1, José Eduardo González-Pastor1
1Department of Molecular Evolution, Centro de Astrobiología (CAB), CSIC-INTA, Carretera de Ajalvir km 4, Torrejón de Ardoz 28850, Madrid, Spain.
Nucleic acids research
|August 28, 2023
概括
转移RNAs (tRNAs) 的Queuosine (Q) 修饰控制了细菌生物膜的形成和毒性. 抑制Q修饰提供了对抗细菌感染的新策略.
科学领域:
- 分子生物学分子生物学
- 微生物学 微生物学
- 生物信息学是一种生物信息学.
背景情况:
- 转移RNA (tRNA) 修改对于精确的蛋白质翻译至关重要.
- 素 (Q) 修改tRNAs影响NAU编码子的翻译,但其生理意义尚未得到充分理解.
- 在细胞功能中Q-修饰的tRNAs (Q-tRNAs) 的精确作用需要进一步的研究.
研究的目的:
- 为了研究tRNAqueuosine (Q) 修饰的生理作用.
- 识别由Q-tRNAs (Q基因) 调节的基因及其相关功能.
- 探索Q修饰作为控制细菌病原体的目标的潜力.
主要方法:
- 开发一种新的生物信息策略来预测Q基因.
- 实验验证Q-tRNA修饰在细菌过程中的作用.
- 利用了各种模型细菌,包括格拉姆阳性和格拉姆阴性物种.
主要成果:
- 生物信息分析显示,Q基因富含与细菌生物膜形成和毒性相关的功能.
- 对tRNA Q-修饰水平的实验操纵显著影响了模型细菌中的生物膜形成和毒性.
- 这项研究提供了通过Q-tRNAs协调生物膜和毒性基因表达的一般机制的第一个证据.
结论:
- TRNA queuosine (Q) 修饰在调节细菌生物膜形成和毒性方面发挥着至关重要的作用.
- 这些发现表明,微生物组内的Q可用性可以影响其整体功能.
- 抑制tRNA Q修饰是一种有希望的治疗策略,用于控制细菌感染和生物膜发育.
相关概念视频
Gene Regulation in Microbial Communities: Quorum Sensing
39
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
39
Biofilms
55
Biofilms are complex communities of microorganisms encased in a self-produced extracellular polysaccharide matrix attached to surfaces. These microbial consortia can include single or multiple species, providing enhanced survival benefits by forming organized, multilayered structures.The formation of biofilms occurs through four key stages: attachment, colonization, development, and dispersal.During attachment, free-swimming planktonic cells adhere to a surface, often facilitated by...
55
Transcription Attenuation in Prokaryotes
15.4K
Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure. Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
15.4K
Bacterial Signaling
32.7K
Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
32.7K
Types of RNA
63.9K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
63.9K
Transfer RNA Synthesis
12.0K
One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
12.0K


