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相关概念视频

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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Ribosomal RNA Synthesis02:53

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Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
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Types of RNA01:20

Types of RNA

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Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
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RNA Splicing01:32

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Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
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相关实验视频

Updated: Jul 17, 2025

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
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A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

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在HSP90和非编码RNA中.

Qing Xu1,2,3,4, Haoduo Qiao1,2,3,4, Yunfei Xu1,2,3,4

  • 1Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha, China.

DNA and cell biology
|August 28, 2023
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概括

热冲击蛋白90 (HSP90) 影响非编码RNA (ncRNA) 的合成和功能. 本综述探讨了ncRNAs如何影响HSP90活动,揭示了一个复杂的调节网络.

关键词:
在HSP90中,它是HSP90的.圆形的RNARNA是一个圆形的RNA.长非编码RNA是什么意思简短的非编码RNA.

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科学领域:

  • 分子生物学分子生物学
  • 细胞生物学 细胞生物学
  • 生物化学 生物化学

背景情况:

  • 热冲击蛋白90 (HSP90) 是对蛋白质折叠和细胞平衡至关重要的分子伴侣.
  • 非编码RNAs (ncRNAs) 调节包括发育,转录和代谢在内的基本细胞过程.
  • ncRNA与包括蛋白质在内的各种分子相互作用,以发挥其功能.

研究的目的:

  • 系统地审查HSP90和ncRNAs之间的多方面的相互作用.
  • 阐明HSP90对不同ncRNAs的合成和功能的影响.
  • 探索ncRNAs对HSP90活动的相互影响.

主要方法:

  • 文献综述和对HSP90和ncRNA相互作用现有研究的系统分析.
  • 整合关于ncRNA生物发生,功能和伴侣调节的数据.
  • 对详细介绍ncrna介导HSP90.0调制的研究进行分析.

主要成果:

  • HSP90影响各种ncRNAs的合成,折叠和功能.
  • ncRNAs直接和间接调节HSP90活动和客户端蛋白相互作用.
  • 一个复杂的相互作用存在,其中HSP90和ncRNA都调节了彼此的生物作用.

结论:

  • HSP90在ncRNAs的生命周期和功能中发挥着重要作用.
  • ncRNAs有助于调节HSP90,影响其伴侣活性.
  • 了解这个HSP90-ncRNA轴对于理解细胞调节和应激反应至关重要.