扩大了Lactiplantibacillus plantarum的遗传编程能力
Marc Blanch-Asensio1, Sourik Dey1, Varun Sai Tadimarri1
1Bioprogrammable Materials, INM-Leibniz Institute for New Materials, Saarbrücken, Germany.
Microbial biotechnology
|August 28, 2023
概括
乳酸菌,特别是乳酸菌植物菌 (Lactiplantibacillus plantarum),对健康有好处,是食品生产的关键. 改善它们的遗传工具对于合成生物学在食品和医疗保健中的应用至关重要.
科学领域:
- 微生物学 微生物学
- 合成生物学 合成生物学
- 基因工程是一种基因工程.
背景情况:
- 乳酸菌是必不可少的微生物,对人类,动物和植物具有共生健康益处.
- 它们在发酵食品工业中发挥着至关重要的作用,并越来越多地被用于医疗保健应用.
- 乳酸菌的基因工程,特别是乳酸菌植物菌 (Lactiplantibacillus plantarum),对于提高它们的能力具有显著的兴趣.
研究的目的:
- 审查现有的遗传部分,用于食品级重组蛋白质的生产,在 Lactiplantibacillus plantarum.
- 确定从过去对L. plantarum的工程工作中吸取的教训.
- 提出开发和优化基因部件用于基因表达调节和生物安全的策略.
主要方法:
- 关于Lactiplantibacillus plantarum遗传部分和工程策略的文献综述.
- 在食品级应用中成功生产重组蛋白质的分析.
- 未来遗传部分开发的投机策略.
主要成果:
- 确定了在L. plantarum.中使食品级重组蛋白质生产成为可能的关键遗传部分.
- 突出了工程L. plantarum的挑战和成功.
- 建议创建新型遗传工具的方法,以加强控制和安全.
结论:
- 对于 Lactiplantibacillus plantarum 的遗传工具包,需要对先进的合成生物学应用进行显著扩展.
- 优化基因部分对于精确控制基因表达和改善生物安全至关重要.
- 对遗传部分发现和工程的进一步研究将释放L. plantarum的全部潜力.
相关概念视频
02:50Transgenic Plants
7.3K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
7.3K
01:20The Central Dogma
21.8K
The central dogma explains the flow of genetic information from DNA nucleotides to the amino acid sequence of proteins.
RNA is the Missing Link Between DNA and Proteins
In the early 1900s, scientists discovered that DNA stores all the information needed for cellular functions and that proteins perform most of these functions. However, the mechanisms of converting genetic information into functional proteins remained unknown for many years. Initially, it was believed that a single gene is...
RNA is the Missing Link Between DNA and Proteins
In the early 1900s, scientists discovered that DNA stores all the information needed for cellular functions and that proteins perform most of these functions. However, the mechanisms of converting genetic information into functional proteins remained unknown for many years. Initially, it was believed that a single gene is...
21.8K
01:25Inducible Operons: lac Operon
48
The lac operon in Escherichia coli is a model for understanding inducible gene regulation and metabolic flexibility. It integrates local control by lactose and global regulation through catabolite repression, enabling E. coli to preferentially metabolize glucose when available and switch to lactose utilization when glucose is scarce.Structure and Function of the lac OperonThe lac operon contains three structural genes: lacZ (β-galactosidase), lacY (lactose permease), and lacA...
48
01:26Transformation
29
Microbial communities are dynamic environments where cell lysis releases free DNA into the surroundings. Other cells can take up this extracellular DNA through a process known as transformation.When a cell incorporates this foreign DNA into its genome, resulting in genetic modification, the process is known as transformation. Cells capable of this process are termed competent. Competence can be natural, as observed in certain bacteria and archaea, or artificially induced in the...
29
00:57Antibiotic Selection
54.7K
Overview
54.7K