Rie1和Sgn1形成一个RNA结合复合体,强制执行介质进入细胞命运决定的过程
Alec Gaspary1, Raphaelle Laureau1, Annie Dyatel1
1Department of Genetics and Development, Hammer Health Sciences Center, Columbia University Irving Medical Center, New York, NY, USA.
The Journal of cell biology
|August 28, 2023
概括
发芽的酵母细胞使用RNA结合蛋白Rie1和Sgn1来控制进入微变的过程. 这些蛋白质在转录后调节主转录因子Ime1,确保准确的细胞命运决定.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 发芽的酵母细胞表现出不同的生理状态,包括快速的植物生殖和子形成以求生存.
- 细胞命运的决定,如进入半转化,是关键的,通常由主转录因子调节.
- 强制执行细胞命运的转录后机制仍然不太了解.
研究的目的:
- 为了识别影响胚芽酵母在转录后水平上介质进入的RNA结合蛋白.
- 阐明RIE1及其约束性伴侣SGN1在调节介质进展中的作用.
主要方法:
- 转发基因选以识别影响介质进入的RNA结合蛋白.
- 生物化学分析以确认RNA结合和与翻译机制的关联.
- 分析蛋白质与蛋白质相互作用和基因表达.
主要成果:
- 一个前进的遗传屏幕确定了RIE1作为一个关键的RNA结合蛋白,影响介质进入.
- Rie1 (与真核细胞启动因子4E1相互作用的RNA结合蛋白) 结合RNA,并在转录后提高Ime1 (Meiosis I表达1) 蛋白质水平.
- 作为Rie1的结合伙伴,Sgn1 (Sporulation-induced gene 1) 也在及时的Ime1表达中发挥作用.
- Rie1和Sgn1的功能独立于细胞大小调节途径.
结论:
- Rie1和Sgn1在转录后作用,通过调节主转录因子Ime1.1,促进介质的进入.
- 这些RNA结合蛋白质在芽的酵母中起到关键的执行和抑制细胞命运决定的作用.
- 这些发现为管理细胞命运决定的转录后控制机制提供了洞察力.
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