生物强化影响番茄果的新陈代谢和转录特征在成熟时
Anton Shiriaev1,2, Stefano Brizzolara1, Carlo Sorce3
1Crop Science Research Center, Sant'Anna School of Advanced Studies, 56127 Pisa, Italy.
Journal of agricultural and food chemistry
|August 28, 2023
概括
番茄中的丰富改变了成熟期间的关键代谢化合物和基因表达,影响了营养质量,但没有影响颜色或乙烯生产.
科学领域:
- 植物生理学 植物生理学
- 生物化学 生物化学
- 分子生物学分子生物学
背景情况:
- 是一种必不可少的微量元素,具有抗氧化特性.
- 了解对水果发育的影响对于提高作物营养价值至关重要.
- 番茄成熟过程涉及复杂的生理和代谢变化.
研究的目的:
- 为了研究丰富对番茄果实成熟的影响.
- 分析对的生理,代谢和分子反应.
- 确定如何影响番茄发育过程中的关键化合物和基因表达.
主要方法:
- 番茄用酸和纳米颗粒 (10毫克L-1) 处理.
- 在成熟过程中监测生理参数 (乙烯,颜色).
- 进行了代谢分析 (主要和次要代谢产物,挥发性化合物).
- 用RNA测序 (RNA-seq) 来分析基因表达.
主要成果:
- 丰富并没有显著改变乙烯生产或水果颜色.
- 在初级和二级代谢中观察到显著的变化.
- 降低了β-胡卜素,增加了宁素和酸,减少了酸.
- 挥发性有机化合物概况发生了变化,阿波卡罗类化合物如β-离子子子发生了变化.
- RNA-seq揭示了对参与荷尔蒙信号传递,二次代谢,黄类生物合成和糖氨基酸降解的基因的影响.
结论:
- 丰富改变了番茄在成熟过程中的生化成分.
- 代谢变化可能与改变的基因表达模式有关.
- 处理提供了一个潜在的策略来调节西红果的质量和生物活性化合物概况.
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