中原激活蛋白KINASE3通过酸化MAPKKK5增强edr1突变体的抗病能力
Wei Wang1,2, Shuling Chen1,3, Guitao Zhong1,3
1State Key Laboratory of Ecological Control of Fujian-Taiwan Crop Pests, Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Plant Immunity Center, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Plant physiology
|August 28, 2023
概括
线素激活蛋白激酶 (MAPK) 级联调节植物免疫力. MPK3在Ser-90中酸化MAPKKK5,增加其稳定性并增强EDR1突变中的植物疾病抵抗力.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物病理学 植物病理学
- 信号传导传导是指信号的传导.
背景情况:
- 线素激活蛋白激酶 (MAPK/MPK) 级联对于调节植物免疫力至关重要.
- 增强的抗病性1 (EDR1),一个MAPKKK,负面控制植物防御反应在Arabidopsis.
- 在edr1突变体中观察到的增强的抗病能力取决于MKK4,MKK5和MPK3,但增加MAPK级联激活的确切机制尚不清楚.
研究的目的:
- 阐明MAPKKK5酸化在Ser-90在植物疾病耐药性中的作用.
- 研究EDR1负调节MAPK级联激活的机制.
- 在植物免疫的背景下确定MPK3和MAPKKK5之间的相互作用.
主要方法:
- 对阿拉比多普西斯突变的遗传分析 (edr1,mapkkk5-2).
- 局部定向的突变生成产生死 (S90A) 和模仿 (S90D) 的MAPKKK5变体.
- 使用Golovinomyces cichoracearum和Pseudomonas syringae pv.的病原体感染试验. 番茄 番茄 番茄 番茄 番茄
- 使用生化技术分析蛋白质稳定性和酸化.
主要成果:
- 在edr1突变体中增强的抗病能力需要MAPKKK5.5.
- 在Ser-90中MAPKKK5的酸化对抗病性至关重要,S90D赋予了增强的抗性.
- 在Ser-90中,MPK3直接化MAPKKK5,增加MAPKKK5的稳定性并促进MAPK级联激活.
- 似乎EDR1通过抑制MPK3介导的MAPKKK5.5的反酸化来负面调节MAPK信号传递.
结论:
- 在Ser-90中MPK3介导的MAPKKK5酸化是增强MAPK级联激活和增强植物抗病能力的关键机制.
- EDR1-MPK3-MAPKKK5调控模块在平衡工厂防御反应方面发挥着重要作用.
- 了解这种信号通路为改善作物对病原体的耐药性提供了潜在的目标.
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