在牙周炎中解读骨质细胞 - 巨细胞交叉的细胞特异效应
Diego Jacho1, Parto Babaniamansour1, Raquel Osorio2,3
1Department of Bioengineering, University of Toledo, Toledo, Ohio, USA.
Tissue engineering. Part A
|August 28, 2023
概括
这项研究揭示了炎症性巨细胞和骨质母细胞在牙周炎中如何相互作用. 在3D矩阵中的接触共培养促进了骨代谢,即使在炎症条件下.
科学领域:
- 生物材料科学 生物材料科学
- 细胞生物学 细胞生物学
- 免疫学 免疫学 免疫学
背景情况:
- 牙周炎通过细菌诱导的炎症来破坏骨重塑.
- 骨质细胞和巨细胞的相互作用是骨平衡的关键 (骨质生成与骨质细胞生成).
- 有限的研究存在于3D生物模拟矩阵中的骨质细胞-巨细胞交叉声.
研究的目的:
- 为了研究骨质母细胞和促炎性巨细胞之间的间接和直接交叉声.
- 在3D组织模拟中阐明这些细胞的协同效应.
- 确定细胞特异相互作用在骨质细胞分化中的作用.
主要方法:
- 为培养骨质母细胞和M1巨细胞创建了一个载有细胞的3D组织模拟.
- 通过条件介质调查的间接交叉通话.
- 在3D支架内分析了接触共同培养的直接协同效应.
主要成果:
- 骨质母细胞的条件介质保持了M1巨细胞的炎症性表型.
- 暴露于巨细胞分泌物的骨质细胞显示RANKL增加和ALP活动降低.
- 3D培养上调的促炎标志物 (TNF-α,IL-1β) 和下调的抗炎标志物 (CCL18).
- 在3D共同培养中的骨质母细胞显示ALP活性增加,而RANKL没有显著的变化,这表明骨类解体.
结论:
- 骨质母细胞部分抑制了巨细胞的骨质母细胞分化,同时保持了它们的炎症状态.
- 在3D矩阵中对骨质母细胞和促炎性巨细胞进行接触共培养,可以促进对骨组织的合成效应.
- 这种3D仿生模型提供了洞察力,了解骨重塑的动态在炎症性骨疾病,如牙周炎.
相关概念视频
01:31Osteoclasts in Bone Remodeling
3.0K
Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...
3.0K
01:17Hormones and Bone Tissue
2.7K
The endocrine system produces and secretes hormones, which interact with the skeletal system. These hormones control bone growth, maintain bone once it is formed, and remodel it.
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
2.7K
01:40Bone Remodeling
38.4K
Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
38.4K