相关实验视频

Updated: Jul 17, 2025

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Published on: March 24, 2017

在 silico 中的表位预测和进化分析揭示了 B 肠道病毒的状突变模式

Hui Wang¹, Yulu Fang¹, Yongtao Jia¹

¹Zhejiang Provincial Key Laboratory of Pathological and Physiological Technology, School of Public Health, Health Science Center, Ningbo University, Ningbo, 315211, China.

|August 28, 2023

概括

肠道病毒B (EVB) 流行是由病毒表位的突变驱动的,而不是受体结合部位. 本研究确定了关键的EVB表位区域和突变热点,为疾病监测和抗体开发提供了洞察力.

科学领域:

病毒学病毒学
分子生物学分子生物学
免疫学免疫学免疫学

背景情况:

肠道病毒B (EVB) 是一种流行的病毒物种,可引起脑膜炎和心肌炎等严重疾病.
之前的研究确定了EVB结构并确定了一些中和抗体表位,但潜在的额外表位及其进化作用仍然不清楚.
了解EVB表位动态对于开发有效的治疗方法和监测流行病至关重要.

研究的目的:

使用生物信息学系统地预测六种代表性EVB血清型的形状表征.
分析EVB表位的分子演变及其与受体结合部位的关系.
调查表位突变在EVB进化和流行病传播中的作用.

主要方法:

基于生物信息学的表位预测算法应用于六种EVB血清型 (E6,E11,E30,CVB1,CVB3,CVB5).
合规表位图和集群. 合规表位图和集群.
使用VP1和基因组序列进行分子进化分析.
与表位和受体结合位相对应的突变分布的分析.

主要成果:

在VP1 BC循环,C终端和VP2 EF循环中聚类,VP1 BC和VP2 EF循环可能调解受体结合.
VP1 C端,VP2 EF循环和VP1 N端被确定为常见的突变热点.
发现突变驱动了进化类的出现.
突变主要发生在表位上或附近,而受体结合部位在很大程度上保持不变.

结论:

EVB通过改变其免疫逃避表位素来促进流行病,同时保留必要的受体结合部位.
皮质突变是EVB分子进化和类型出现的关键驱动因素.
这项生物信息学研究为EVB流行病监测和向治疗抗体的开发提供了有价值的数据.

更多相关视频

Detection of Neutralization-sensitive Epitopes in Antigens Displayed on Virus-Like Particle VLP-Based Vaccines Using a Capture Assay

Detection of Neutralization-sensitive Epitopes in Antigens Displayed on Virus-Like Particle VLP-Based Vaccines Using a Capture Assay

Published on: February 10, 2022

Alternative In Vitro Methods for the Determination of Viral Capsid Structural Integrity

Alternative In Vitro Methods for the Determination of Viral Capsid Structural Integrity

Published on: November 16, 2017

相关概念视频

Viral Mutations

Viral Mutations

A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material...

Viral Structure

Viral Structure

Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.