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聚合力调节的雅丁丝-Arp2/3复合相互作用主导自我适应的细胞迁移.
Xindong Chen1,2, Yuhui Li3, Ming Guo4
1Institute of Biomechanics and Medical Engineering, Department of Engineering Mechanics, Tsinghua University, Beijing 100084, China.
概括
细胞通过新的"阻力适应推进"理论来适应它们的运动,以适应复杂的环境. 该模型解释了蛋白质相互作用如何使细胞能够在迁移过程中克服障碍物并有效利用资源.
科学领域:
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
- 计算生物学 计算生物学
背景情况:
- 细胞迁移对于癌症入侵和免疫反应至关重要.
- 了解推动细胞适应异质微环境的分子机制是有限的.
- 在细胞迁移中对毫秒级蛋白质动态的实验探测具有挑战性.
研究的目的:
- 建立一个理论和多尺度建模系统,解释细胞如何适应迁移到复杂的细胞外微环境 (ECM).
- 阐明控制自我适应细胞推进和形态变化的分子相互作用.
主要方法:
- 开发了一个时空空间.
- 电阻适应式推进系统
- 一个理论. 一个理论.
- 创建了一个从蛋白质相互作用到整个细胞行为的多尺度动态建模系统.
- 分析了Arp2/3复合体和actin线索动态的作用.
主要成果:
- 细胞通过阻力触发的正负反机制自我适应推进力,以异质ECM.
- 积极的反,由动丝曲和Arp2/3结合驱动,增强推进.
- 负反阻碍了在高阻力区域的分支光纤组件,使绕障碍物成为可能.
- 细胞迁移速度表现出由于细胞骨重塑而导致的时间歇斯底里.
- 方向迁移取决于ECM的刚性和局部actin聚合率.
结论:
- 聚合力,活性丝和Arp2/3复合结合之间的相互作用决定了自我适应的细胞迁移.
- 开发的理论和模型为复杂的ECM中细胞运动动态提供了预测性见解.
- 协同反机制赋予强大的,灵活的迁移和高效的蛋白质利用.
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