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来自海洋衍生真菌的新二次代谢物 Phomopsis lithocarpus FS508 FS508

Zi-Ling Tan^1,2, Yu-Chan Chen², Jian-Peng Zhang^1,2

¹School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China.

Journal of Asian natural products research

|August 28, 2023

概括

研究人员使用一株多种化合物 (OSMAC) 策略探索了Phomopsis lithocarpus FS508的二次代谢物. 这导致了新化合物的发现和细胞毒性和抗炎作用的评估.

科学领域:

自然产品化学自然产品化学
药用化学医学化学
微生物学微生物学

背景情况:

一个菌株-许多化合物 (OSMAC) 策略是一种强大的方法,用于从微生物中发现新的二次代谢物.
菲莫普西斯物种是众所周知的多种生物活性化合物的生产者.

研究的目的:

使用OSMAC策略研究由Phomopsis lithocarpus FS508产生的二次代谢产物.
为了分离和描述新的和已知的化合物.
评估单独的化合物对细胞毒性和抗炎活性.

主要方法:

在特定条件下发酵Phomopsis lithocarpus FS508.
使用色谱技术分离和净化二次代谢产物.
使用先进的光谱方法 (NMR,IR,UV,MS) 阐明化合物的结构.
在体外测试以评估对HepG-2细胞系和抗炎作用的细胞毒性活性.

主要成果:

一种新的二次代谢产物,4-甲基-3-[4-(乙基) -3-甲基-2-丁]酸 (1),被分离和表征.
还从发酵中确定了11种已知的化合物.
化合物3和9对HepG-2细胞系表现出强烈的细胞毒性.
化合物2,3,和12表现出显著的抗炎活性.

结论:

通过OSMAC的策略,从Phomopsis lithocarpus FS508.8中发现了一种新的化合物.
隔离的化合物具有有前途的细胞毒性和抗炎性质,需要进一步研究潜在的治疗应用.

关键词:

海洋真菌海洋真菌这是OSMAC的OSMAC.菲莫普西斯 (Phomopsis lithocarpus) 是一种类型的植物.这是一种抗炎药物.细胞毒性细胞毒性

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