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有效化学合成N2 - 修改型关诺辛酸衍生物的协议:用于mRNA标记的多功能探针

Annamalai Senthilvelan¹, Muthian Shanmugasundaram¹, Anilkumar R Kore¹

¹Life Sciences and Laboratory Products Group, Thermo Fisher Scientific, Austin, Texas.

Current protocols

|August 28, 2023

概括

这项研究介绍了一种简单的合成N2-修饰瓜诺辛核酸的方法. 这种高效的工艺产生高纯度的化合物,对各种生物化学应用有价值.

科学领域:

有机化学有机化学
核酸化学核酸化学
生物化学生物化学

背景情况:

瓜诺辛核酸是核酸和信号通路中的基本构建块.
修改瓜诺辛核酸可以改变它们的生物活性和研究中的实用性.
现有的合成方法可能缺乏效率,可靠性或可扩展性.

研究的目的:

开发一种简单,可靠和高效的方法来合成N2修饰的瓜诺辛核酸.
为了生产特定的N2修饰的瓜诺辛-5'-O-单酸盐,二酸盐和三酸盐衍生物.
在合成过程中实现高纯度和中等产量.

主要方法:

在水性甲醇中,瓜诺辛核酸的外循环氨基和3-[(基基) 氨基] propionaldehyde 之间的凝结反应.
通过使用氨酸化来减少中间体.
合成的N2-修饰的瓜诺辛核酸的净化和表征.

主要成果:

成功合成了N2-[基-N-() 碳酸盐]-瓜诺辛-5'-O-单酸盐, -二酸盐和 -三酸盐.
合成N2-[基-N-(基) 碳酸盐]-N7-甲基-瓜诺辛-5'-O-二酸盐.
获得的产品具有高纯度 (>99.5%) 和中等产量.

结论:

描述的方法为N2修饰的瓜诺辛核酸合成提供了可靠的途径.
该协议是有效和可靠的生产有价值的核酸类比物.
合成化合物的高纯度使它们适合进一步的生物化学研究.

关键词:

修改为N2的瓜诺辛是什么?在 mRNA 封顶上.核酸核酸是一种核酸.氧核酸类的部分.治疗应用治疗应用

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