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miR-128-3p通过调节血管内皮生长因子C来增加鼻癌的辐射敏感性

Lisha Peng¹, Yong Wang¹, Jie Luo¹

¹Department of Radiotherapy, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032, China.

Pathology, research and practice

|August 28, 2023

概括

微RNA-128-3p通过向VEGFC,增强鼻癌的辐射敏感性. 这一发现提供了一个新的治疗策略,以克服NPC患者的辐射耐药性.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
放射治疗研究放射治疗研究

背景情况:

鼻癌 (NPC) 表现出对放射治疗的耐药性.
确定增强辐射敏感性的新目标对于改善NPC治疗结果至关重要.

研究的目的:

研究微RNA-128-3p (miR-128-3p) 在调节鼻癌 (NPC) 放射敏感性的作用.
阐明潜在的分子机制,特别是miR-128-3p和血管内皮生长因子C (VEGFC) 之间的相互作用.

主要方法:

在NPC细胞系 (6-10B,C666-1) 中利用细胞转染 (模仿,抑制剂) 和基因沉默 (shRNA).
通过RT-qPCR和西方布洛特量化基因和蛋白质表达.
评估细胞功能,包括增殖 (CCK-8),DNA损伤 (彗星测定),细胞灭绝 (流细胞计,TUNEL染色),以及小鼠体内瘤生长.

主要成果:

miR-128-3p直接针对并负面调节VEGFC.
过度表达miR-128-3p或VEGFC倒置抑制了NPC细胞增殖,诱导了DNA损伤和亡,并增加了放射敏感性.
相反,miR-128-3p抑制或VEGFC上调促进了扩散和放射电阻.
miR-128-3p对NF-κB信号通路进行调节 (p-P50/p-P65/p-IκB).
在体内研究证实了miR-128-3p对NPC生长的抑制作用及其在促进DNA损伤和亡中的作用.

结论:

miR-128-3p/VEGFC轴在调节NPC辐射敏感性方面发挥着重要作用.
准miR-128-3p通路是一个有希望的治疗策略,以克服鼻癌的辐射抵抗.

关键词:

在 MiR-1283p 里面.鼻癌是指鼻癌.核武器的扩散辐射疗法辐射疗法血管内皮生长因子C 血管内皮生长因子C

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