相关实验视频
Updated: Jul 17, 2025

09:04
Measurement of T Cell Alloreactivity Using Imaging Flow Cytometry
Published on: April 19, 2017
13.6K
人类寡细胞上的ALCAM调解了CD4 T细胞粘附
Hélène Jamann1,2, Haritha L Desu1, Qiao-Ling Cui3
1Neuroimmunology unit, Centre de Recherche du Centre Hospitalier de l'Université de Montréal (CRCHUM), Montreal, H2X 0A9, Canada.
Brain : a journal of neurology
|August 28, 2023
概括
激活白细胞细胞粘附分子 (ALCAM) 在人体寡聚基细胞上促进多发性硬化症中破坏性T细胞相互作用. 阻断ALCAM通过减少T细胞粘附和对髓化过程的损伤来提供神经保护.
科学领域:
- 神经免疫学 神经免疫学
- 细胞和分子神经科学
背景情况:
- 多发性硬化症 (MS) 是一种慢性神经炎症性疾病,导致脱和神经轴损伤.
- 目前,还没有一种疗法可以保护寡聚基细胞及其髓层免受免疫中介损伤.
- 支持炎症的T辅助17 (Th17) 细胞在MS中直接损伤寡干细胞,但底层的分子机制尚不清楚.
研究的目的:
- 研究细胞粘附分子 (CAMs) 在成熟的寡细胞与Th17细胞相互作用中的作用.
- 通过了解这些有害相互作用来确定MS中神经保护的潜在治疗点.
主要方法:
- 在人类和小鼠中枢神经系统组织上进行单细胞RNA测序,流细胞计和免疫光检测.
- 对暴露于促炎性细胞因子和激活的T细胞的人类初级寡腺细胞的分析.
- 在体外实验室实时成像,沉默和阻断实验中,针对Th17-oligodendrocyte相互作用中的MCAM和ALCAM.
主要成果:
- 人类和小鼠的成熟寡类细胞表达黑色素瘤细胞粘附分子 (MCAM) 和激活白细胞细胞粘附分子 (ALCAM).
- 炎症和T细胞接触降低了MCMAM的调节,但并没有降低ALCAM对人类的寡细胞的调节.
- 沉默或阻断ALCAM显著降低了Th17细胞粘附和细胞毒性对人类寡细胞的作用,保护了它们的过程.
结论:
- 人类的寡干细胞表达MCAM和ALCAM,通过炎症和T细胞接触进行差异调节.
- 阿尔卡姆作为Th17细胞的配体,调解它们的粘附和随后对寡头质细胞的损伤.
- 在多发性硬化症中,ALCAM是神经保护的有希望的治疗点.
更多相关视频
相关概念视频
Immunoglobulin-like Cell Adhesion Molecules
3.3K
Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
3.3K
T Cell Activation and Clonal Selection
804
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Naive T cells that have not yet encountered an antigen express two primary CD...
804
Cell Adhesion Molecules - Types and Functions
6.8K
Cell adhesion molecules (CAMs) are pivotal to multicellularity and the coordinated functioning of tissues and organ systems. They enable physical interactions between cells and provide mechanical strength to tissues. They also function as receptors for signal transmission across the plasma membrane. The CAMs are broadly classified into four families - integrins, cadherins, selectins, and immunoglobulin-like CAMs (IgCAMs).
CAM Families
The Integrin family of proteins is primarily involved...
CAM Families
The Integrin family of proteins is primarily involved...
6.8K
Intracellular Signaling Affects Focal Adhesions
2.7K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Some...
2.7K
Cadherins in Tissue Organization
3.0K
The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
Cell Sorting During Development
Cell sorting plays an...
Cell Sorting During Development
Cell sorting plays an...
3.0K
Anchoring Junctions
3.8K
Anchoring junctions are multiprotein complexes that help cells connect to other cells and the extracellular matrix. Anchoring junctions are present on the lateral and basal surfaces of cells, providing strong and flexible connections. Focal adhesions are often formed due to cell interactions with the ECM substrata, which initiate signal transduction via kinase cascades and other mechanisms. Together, they provide stability and tissue integrity. There are three types of anchoring junctions:...
3.8K

