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来自埃索诺菲尔的加勒丁-10可调节母线金属蛋白酶表达在球状皮虫泡中的表达

Takahiko Sato¹, Takahito Chiba¹, Takeshi Nakahara²

¹Department of Dermatology and Plastic Surgery, Akita University Graduate School of Medicine, Akita, Japan.

Journal of dermatological science

|August 28, 2023

概括

球状皮虫 (BP) 泡中的埃索诺菲尔细胞经历细胞死亡,释放出加勒-10. 这种蛋白质可以通过刺激角质细胞和纤维细胞产生矩阵金属蛋白酶 (MMPs) 来驱动囊泡形成.

科学领域:

皮肤病学皮肤病学
免疫学免疫学免疫学
细胞生物学细胞生物学

背景情况:

球状皮炎 (BP) 是一种自身免疫性水泡性疾病,其特征是异osinophil 透.
盖莱克-10在埃索诺菲尔中丰富,并在埃索诺菲尔细胞外陷细胞死亡 (EETosis) 期间释放.

研究的目的:

为了研究EETosis和galectin-10在BP病变发生中的作用.
探索加勒-10促进囊泡形成的机制.

主要方法:

免疫光学和电子显微镜证实了BP泡中EETosis和galectin-10的存在.
ELISA测量了血清和囊泡液中的加勒-10水平.
免疫组织化学,实时PCR和西式涂抹评估了 in vitro 和 in vivo 中的矩阵金属蛋白酶 (MMP) 表达和信号通路.

主要成果:

在BP水泡中发现了表现出EETosis和释放galectin-10的eosinophils.
与血清相比,在泡液中发现了更高的加勒-10度.
盖莱克-10通过p38 MAPK,ERK和JNK通路在角质细胞和纤维细胞中刺激MMP的产生.

结论:

乙氨基经历EETosis,在BP水泡中释放甲烯-10.
加勒-10通过诱导皮肤细胞中MMP的产生,有助于BP泡的形成.

关键词:

这种类型的类类虫.乙氨基酸细胞外细胞陷细胞死亡 (EETosis)埃索诺菲尔细胞是什么意思盖莱克-10 是一种蛋白质.矩阵金属蛋白酶是一种金属蛋白酶.

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