对SBF-SEM样本的最小树脂嵌入减少了充电,并促进了寻找感兴趣的表面连接区域
Barbora Konopová1,2, Jiří Týč3
1Institute of Entomology, Biology Centre CAS, České Budějovice, Czech Republic. barbora.konopova@entu.cas.cz.
Frontiers in zoology
|August 28, 2023
概括
对于串行块面扫描电子显微镜 (SBF-SEM) 的最小树脂 (MR) 嵌入,最大限度地降低了充电并简化了感兴趣区域的定位. 这种技术提高了对生物超结构研究的成像质量和效率.
科学领域:
- 电子显微镜电子显微镜
- 生物成像技术 生物成像技术
- 细胞生物学 细胞生物学
背景情况:
- 高分辨率的3D成像对于理解细胞和器官超结构至关重要.
- 序列块面扫描电子显微镜 (SBF-SEM) 能够以纳米分辨率对大型生物样本进行3D成像.
- 由于过多的树脂,充电效应和模糊的形态阻碍了SBF-SEM的质量和导航.
研究的目的:
- 介绍SBF-SEM样品制备工作流程,使用最小的树脂 (MR) 嵌入.
- 为了解决SBF-SEM中的收费和兴趣区域 (ROI) 定位挑战.
- 为了提高SBF-SEM的成像质量和效率.
主要方法:
- 开发并应用了用于SBF-SEM样品制备的最小树脂 (MR) 嵌入方法.
- 用小关节动物和鱼作为示例样本.
- 在成像室放置之前,证明了样品修剪.
主要成果:
- 该MR嵌入方法有效地解决了SBF-SEM的收费和ROI本地化问题.
- 表面结构在没有树脂掩饰的情况下得到了清晰的成像,允许精确的修剪.
- 缺少或减少充电可以实现最佳的高真空成像,提高分辨率,信号噪声比和采集速度.
结论:
- 在SBF-SEM中嵌入MR可以最大限度地降低收费并简化ROI目标.
- 该技术很容易实施,不需要特殊的设备或技能.
- 嵌入MR可以节省时间和精力,从而获得高质量的数据,特别是对于表面连接或树脂遮蔽的样品.
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