在动脉样硬化中探索自中介性巨细胞灭的生物标志物
Rongyuan Yang1, Dawei Wang2, Yu Ding3
1Department of cardiovascular disease, The Second Clinical School of Medicine, Guangzhou University of Chinese Medicine, Zhuhai, China.
Cell biology international
|August 29, 2023
概括
这项研究确定了调节动脉样硬化中的巨细胞自和热的关键基因. 这些发现通过了解免疫细胞参与,为动脉样硬化提供了潜在的治疗点.
科学领域:
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
- 心血管研究研究心血管研究
背景情况:
- 动脉样硬化涉及复杂的细胞过程,包括巨细胞功能障碍.
- 自和热是关键的细胞事件,涉及动脉样硬化病原.
- 了解巨细胞自和烧之间的相互作用对于开发向疗法至关重要.
研究的目的:
- 为了研究巨细胞自与动脉样硬化相关的亡的作用.
- 为了确定参与这个过程的关键基因和途径.
- 探索已识别的基因作为诊断或治疗标记物的潜力.
主要方法:
- 利用基因表达总量 (GEO) 数据集 (GSE100927,GSE159677) 进行差异性基因表达分析,基因本体学 (GO),基因和基因组的京都百科全书 (KEGG),CIBERSORT,权重相关联网络分析 (WGCNA),接收器操作特征 (ROC),基因组丰富分析 (GSEA) 和相关性分析.
- 使用STRING构建蛋白质与蛋白质相互作用 (PPI) 网络,并使用Cytoscape进行分析.
- 采用NetworkAnalyst来探索转录因子,药物和组织共同表达网络;对GSE159677进行单细胞分析,并在THPA人体样本中验证.
主要成果:
- 鉴定了110个差异表达的自相关基因 (DEATGs),在自,发酵体和 lysosome 途径中进行丰富.
- 发现了35个与免疫相关的DEATG,富含免疫效应过程和互白素-6信号;确定了6个具有高预测值 (AUC=0.961) 的枢纽基因,用于巨细胞自相关的热亡.
- 发现了3个中枢基因,它们调解了巨细胞的自相关性热,单细胞分析证实了它们在人体样本中的分布和相关性.
结论:
- 确定了特定的枢纽基因,这些基因在调节动脉样硬化中的巨细胞自中介性铁灭亡方面至关重要.
- 这些已识别的基因具有显著的预测价值,并且与参与动脉样硬化的关键免疫细胞相关.
- 这些发现为动脉样硬化的分子机制提供了新的见解,并提出了潜在的治疗点.
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