与牛概念的延长相关的转录组变化II:在受精后的第17天,子宫内膜中的差异表达的转录
P M Peixoto1, J J Bromfield2, E S Ribeiro3
1Department of Large Animal Clinical Sciences, D. H. Barron Reproductive, and Perinatal Biology Research Program, University of Florida, Gainesville, FL 32610.
Journal of dairy science
|August 29, 2023
概括
乳牛子宫内膜基因表达在怀孕第17天的概念大小上有显著差异. 小概念引发了最重要的变化,影响了免疫反应途径.
科学领域:
- 生殖生物学 生殖生物学
- 基因组学就是基因组学.
- 免疫学 免疫学 免疫学
背景情况:
- 子宫内膜受容性对于牛的怀孕确立至关重要.
- 孕胎-子宫内膜相互作用会影响怀孕早期的结果.
- 了解子宫内膜中的基因表达变化是提高生殖效率的关键.
研究的目的:
- 在怀孕第17天,对乳牛的子宫内膜转录组进行表征.
- 为了研究概念长度和子宫内膜基因表达之间的关系.
- 确定关键的分子通路,参与在牛早期怀孕的识别.
主要方法:
- 在AI后17天,对荷尔斯坦奶牛 (n=48) 的子宫内膜组织进行转录组分析.
- 概念按长度分类:小 (1.2-6.9厘米),中等 (10.5-16.0厘米) 和大 (18.0-26.4厘米).
- 差异基因表达分析和途径分析使用Affymetrix基因芯片牛群阵列和英才途径分析.
主要成果:
- 大型和小型概念群 (LvsS) 之间有235个成绩单的差异,大型和中型之间有21个成绩单 (LvsM),中型和小型之间有94个成绩单 (MvsS).
- 在LvsS和MvsS的比较中,与免疫相关的途径 (抗原呈现,Th1/Th2激活) 是突出的.
- 干扰素α和γ被确定为关键的上游调节剂,影响白细胞和T淋巴细胞活性.
结论:
- 大多数子宫内膜转录基因差异在具有小子宫的奶牛和中等或大子宫的奶牛之间观察到.
- 这些变化主要与母体免疫反应的调节有关.
- 概念体大小显著影响子宫内膜转录组,突出其在怀孕建立中的作用.
更多相关视频
08:00Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay
Published on: June 16, 2021
4.4K
09:11Isolation of Small Preantral Follicles from the Bovine Ovary Using a Combination of Fragmentation, Homogenization, and Serial Filtration
Published on: September 27, 2022
3.6K
相关概念视频
01:19Secretory Phase
828
The secretory phase of the menstrual cycle, spanning from day 14 to 28 in a typical 28-day cycle, is a period of significant physiological changes in the female reproductive system. This phase commences immediately after ovulation and is characterized by the preparation of the endometrium for potential embryo implantation.
Following ovulation, the corpus luteum, a temporary endocrine structure, produces progesterone and estrogens. These hormones stimulate the growth and coiling of endometrial...
Following ovulation, the corpus luteum, a temporary endocrine structure, produces progesterone and estrogens. These hormones stimulate the growth and coiling of endometrial...
828
02:01pre-mRNA Processing
53.0K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
53.0K