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患有持续的亚临床子宫内膜炎的产后奶牛的循环microRNA资料的表征

Gonçalo Pereira¹, Gilles Charpigny², Yongzhi Guo³

¹CIISA-Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, Portugal; Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), 1300-477 Lisbon, Portugal.

Journal of dairy science

|August 29, 2023

概括

乳牛的亚临床子宫内膜炎 (SCE) 与不同的循环microRNA (miRNA) 档案有关. 特定的miRNAs,如miR-425-3p和miR-2285z,显示出这种降低生育能力的疾病的非侵入性诊断的潜力.

科学领域:

兽医医学兽医医学兽医医学
生殖生物学生殖生物学
基因组学就是基因组学.

背景情况:

亚临床子宫内膜炎 (SCE) 是乳牛中流行的一种产后子宫炎症,显著影响生育能力.
目前用于SCE的诊断方法,如子宫细胞学和活检,是侵入性的,可以通过识别基于血液的生物标志物来改进.
已知微RNAs (miRNAs) 在各种疾病过程中发挥作用,使它们成为诊断标记物的潜在候选者.

研究的目的:

为了研究患有亚临床子宫内膜炎 (SCE) 的奶牛中无细胞循环的miRNA概况.
为了确定潜在的基于血液的生物标志物,用于持续性SCE的非侵入性诊断.
探索SCE奶牛子宫内膜中循环miRNA和差异表达基因之间的关系.

主要方法:

一项涉及健康 (n=6) 和SCE (n=11) 产后奶牛的病例控制研究,在产后21天和44天 (DPP) 取样.
提取无细胞血miRNAs,然后进行RNA测序和差异丰度分析,使用DESeq2.
使用miRWalk数据库进行miRNA相互作用转录的in silico预测.

主要成果:

主要组件分析揭示了子宫健康表型 (健康与SCE) 的明显聚类.
总共有34个循环的miRNA在健康和SCE奶牛之间差异丰富 (SCE中的12个较低,22个较高).
循环中的miRNAs,特别是miR-425-3p和miR-2285z,在区分SCE和健康奶牛方面表现出高的区分能力.

结论:

乳牛的亚临床子宫内膜炎与独特的循环miRNA特征有关,这表明与子宫内膜炎的系统性联系.
循环的无细胞miRNAs,特别是miR-425-3p和miR-2285z,显示出作为持续性SCE的非侵入性诊断生物标志物的希望.
识别这些miRNA生物标志物可以显著提高SCE的早期检测和管理,从而提高奶牛的生育能力.

关键词:

血液中的微RNAs.奶牛牛奶牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛亚临床子宫内膜炎是什么

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...