相关实验视频

Updated: Jul 17, 2025

Defining Substrate Specificities for Lipase and Phospholipase Candidates

Defining Substrate Specificities for Lipase and Phospholipase Candidates

Published on: November 23, 2016

非特异性脂酶C4水解酸和酸糖,促进了菜种的生长和产量

Bao Yang¹, Jianwu Li^2,3, Jiayu Yan¹

¹National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070, China.

Journal of integrative plant biology

|August 29, 2023

概括

兰子中的非特异性脂酶C4 (NPC4) 从膜脂中释放酸盐,增强植物生长和种子产量. 改变NPC4水平会对植物发育和种子生产产生重大影响,尤其是在酸盐有限的条件下.

科学领域:

植物生物学植物生物学
生物化学生物化学
分子遗传学分子遗传学

背景情况:

对于植物生长至关重要,脂是细胞的主要沉积物.
了解植物如何管理对于提高作物产量和营养使用效率至关重要.

研究的目的:

为了研究大麻 (Brassica napus) 中非特异性PHOSPHOLIPASE C4 (NPC4) 在酸盐代谢中的作用及其对植物生长和种子产量的影响.
阐明BnaNPC4的酶活性及其对脂质代谢和酸盐可用性的影响.

主要方法:

利用CRISPR/Cas9基因编辑来创建Brassica napus中的NPC4淘汰和过度表达线.
进行了体外酶分析,以确定BnaNPC4基质的特异性.
分析了脂含量的变化,与脂质代谢和酸盐运输相关的基因表达,以及在植物中具有改变NPC4功能的自由无机酸盐水平.

主要成果:

淘汰BnaNPC4导致脂积累增加,植物生长减少.
过度表达BnaNPC4导致脂含量降低,增强植物生长,增加种子生产.
BnaNPC4被证明可以解基脂和糖脂,影响根和叶的脂质配置,特别是在缺乏的情况下.
BnaNPC4的过度表达调节了参与脂质代谢,酸盐释放和运输的基因,增加了叶子无机酸盐的含量.

结论:

BnaNPC4在菜种子中从膜脂中释放酸盐方面发挥着关键作用.
脂的BnaNPC4介导的水解增强了酸盐的可用性,促进了植物生长和种子产量,特别是在酸盐有限的条件下.

关键词:

无机是一种无机.非特定的C4脂酶.酸盐缺乏症是因为缺乏酸盐.脂质是一种脂质.使用的效率提高了的使用效率.菜种子菜种子

更多相关视频

Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes

Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes

Published on: March 14, 2021

Detection of Phospholipase C Activity in the Brain Homogenate from the Honeybee

Detection of Phospholipase C Activity in the Brain Homogenate from the Honeybee

Published on: September 14, 2018

相关概念视频

IP3/DAG Signaling Pathway

IP3/DAG Signaling Pathway

Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and...

Phosphoinositides and PIPs

Phosphoinositides and PIPs

Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...

Synthesis of Phosphatidylcholine in the ER Membrane

Synthesis of Phosphatidylcholine in the ER Membrane

The ER synthesizes lipids for building cell membranes and performing cellular functions such as energy storage and signaling. The lipid synthesis machinery embedded in the ER membrane primarily collects all reactants from the cytosol. Following synthesis, the secretory pathway and the ER contact sites distribute these lipids to other cellular organelles. Additionally, the energy-rich triacylglycerides are transported from the ER via lipid droplets.
The major components of all eukaryotic cell...

Amplifying Signals via Second Messengers

Amplifying Signals via Second Messengers

Many receptor binding ligands are hydrophilic; they do not cross the cell membrane but bind to cell-surface receptors. Thus, their message must be relayed by second messengers present in the cell cytoplasm. There are several second messenger pathways, each with its own way of relaying information. For example, the G protein-coupled receptors can activate both phosphoinositol and cyclic AMP (cAMP) second messenger pathways. The phosphoinositol pathway is active when the receptor induces...

C4 Pathway and CAM

C4 Pathway and CAM

Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...

Cell Signaling in Plants

Cell Signaling in Plants

Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...