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共素调节自发信号传递和耳外毛细胞中 afferent 内置的成熟

Yang Yang¹, Kaitlin Murtha¹, Leslie K Climer¹

¹Department of Biology, Baylor University, Waco, TX, USA.

The Journal of physiology

|August 29, 2023

概括

模素 (OCM) 调节在发育耳外毛细胞 (OHC) 中的活性. 它的缺失延迟了OHC afferent突触的成熟,影响了听力发育.

科学领域:

神经科学是一个神经科学.
听觉生理学听觉生理学
发展生物学发展生物学

背景情况:

耳外毛细胞 (OHCs) 对于听觉灵敏度和频率选择性至关重要.
OHC afferent连接的成熟依赖于发育过程中的自发 (Ca2+) 活性.
已知结合蛋白的甲摩杜林 (OCM) 调节新生儿OHC中的Ca2+信号传递.

研究的目的:

研究OCM在调节OHC在发育过程中的自发Ca2+活动中的作用.
确定OCM是否影响OHC afferent连接的成熟.

主要方法:

利用基因编码的Ca2+传感器 (GCaMP6s),表达在野生类型和Ocm淘汰赛小鼠的OHC中.
在Ocm+/+和Ocm-/- OHC中比较自发的Ca2+活性和纯能受体表达.
在听力开始之前评估了附带突触成熟,包括带突触和附带纤维.

主要成果:

在出生后的Ocm-/- OHC中观察到自发Ca2+活性增加和纯能受体的上调.
缺少OCM导致OHCs的附带突触成熟延迟.
在听力发作之前,OCM-/- OHCs显示了更多的带状突触和 afferent 纤维.

结论:

在正在发育的尾中,OCM调节自发的Ca2+信号传递.
OCM对于OHC afferent内置的及时成熟至关重要.
在OHCs发育过程中,OCM影响Ca2+活动,Ca2+通道表达和附带连接,影响听觉系统发育.

关键词:

EF-手的结合蛋白质可以结合.在康莫杜林 (OCM) 上.外部毛细胞 (OHC)预突触带带是什么意思纯能受体的纯能受体自发的活性活动.电压关闭通道的通道.

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