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超高分辨率的第二子生成成像与多焦结构照明显微镜

Chenshuang Zhang¹, Fangrui Lin¹, Yong Zhang¹

¹Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, People's Republic of China.

|August 29, 2023

概括

一种新的超高分辨率成像技术,多焦结构照明显微镜-SHG (MSIM-SHG),克服了生物成像的衍射极限. 这种方法增强了组织中细胞结构和原纤维的可视化.

科学领域:

生物医学成像技术生物医学成像技术
光学显微镜的使用方法
生物物理学的生物物理.

背景情况:

第二和生成 (SHG) 成像提供了无外源标签的生理结构的非侵入性可视化.
传统的SHG成像分辨率从根本上受到光学衍射的限制.
克服衍射极限对于详细的生物和材料科学研究至关重要.

研究的目的:

通过将SHG与多焦结构化照明显微镜 (MSIM) 结合起来,开发一种超高分辨率的成像技术.
证明新型MSIM-SHG方法在生物组织和细胞的高分辨率成像方面的能力.
评估MSIM-SHG对量化各种组织中的原纤维对齐的实用性.

主要方法:

多焦结构照明显微镜-SHG (MSIM-SHG) 的开发.
对氧化 (ZnO) 颗粒进行成像,以确定成像分辨率.
应用MSIM-SHG来量化卵巢,肌肉,心脏,脏和软骨组织中的原纤维对齐.

主要成果:

MSIM-SHG在半最大 (fwhm) 时实现了 147 ± 13 nm 的横向全宽度和 493 ± 47 nm 的轴向fwhm.
该技术成功量化了多种生物组织类型中的原纤维对齐.
证明了在各种组织中识别特定的原特性的可行性.

结论:

MSIM-SHG成功地突破了SHG成像的衍射极限,使得超分辨率成为可能.
该方法提供生物结构的高分辨率成像,并有效用于原蛋白分析.
作为先进的临床诊断和基本生物学研究的工具,MSIM-SHG具有显著的潜力.

关键词:

多焦点结构化照明显微镜第二声的第二声生成.超高分辨率成像成像技术

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