人类细胞在单个粒子水平上快速内化纳米粒子
Ceri J Richards1,2, Thomas C Q Burgers2, Rifka Vlijm2
1Pharmaceutical Analysis, Groningen Research Institute of Pharmacy, University of Groningen, 9713 AV Groningen, The Netherlands.
ACS nano
|August 29, 2023
概括
大多数与细胞相互作用的纳米颗粒消化,但内部化颗粒在几秒内迅速进入. 这种快速吸收表明了先前存在的或新的内细胞通路,提供了对纳米粒子细胞进入和贩运的定量见解.
科学领域:
- 细胞生物学 细胞生物学
- 纳米技术 纳米技术
- 生物物理学的生物物理.
背景情况:
- 纳米粒子吸收对于纳米医学和纳米安全至关重要.
- 纳米粒子内部化的定量方面,如速率和数量,研究不足.
- 理解纳米粒子-细胞相互作用需要详细的动力学分析.
研究的目的:
- 量化研究人类胚胎细胞纳米粒子内化运动的动力学.
- 为了确定纳米粒子吸附,脱附和内部化的时间尺度.
- 探索参与快速纳米粒子吸收的机制和途径.
主要方法:
- 活细胞共聚焦和超分辨率刺激排放枯竭 (STED) 光显微镜.
- 在细胞膜上追踪40-200nm碳酸聚烯纳米颗粒.
- 监测颗粒脱离,内部化和随后的细胞内贩运.
主要成果:
- 大多数吸附的纳米颗粒从细胞膜中脱离.
- 大多数内化纳米粒子在几秒钟内被吸收,无论大小如何.
- 一些纳米粒子在几十秒内从细胞中迅速脱离.
结论:
- 快速纳米粒子内部化表明正在进行的内细胞事件或新的途径的参与.
- 定量运动数据为纳米粒子-细胞相互作用提供了关键的见解.
- 这项研究强调了纳米粒子在细胞水平上贩运的动态性质.
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