现在的趋势和前景在微生物生物转换的类固醇
1G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center "Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences", Pushchino, Russia. donova@ibpm.pushchino.ru.
Methods in molecular biology (Clifton, N.J.)
|August 29, 2023
概括
微生物类固醇生物技术使用微生物生产有价值的类固醇前体,用于药物和其他行业. 代谢工程和合成生物学方面的进步正在为类固醇生产创造改进的微生物生物催化剂.
科学领域:
- 生物技术是生物技术.
- 微生物学 微生物学
- 合成生物学 合成生物学
- 代谢工程是代谢工程.
背景情况:
- 类固醇的微生物转化是工业类固醇前体 (synthon) 生产的基础.
- 这些合成子对于合成用于医学,制药,食品和农业的多种类型的类固醇和印丹异oprenoids至关重要.
- 了解微生物类固醇降解和基因技术的进步,已经彻底改变了类固醇生物技术.
研究的目的:
- 审查微生物类固醇生物技术当前的趋势和进展.
- 突出代谢工程和合成生物学在改善生物催化剂中的应用.
- 探索创建用于类固醇生产的新型全细胞微生物生物催化剂.
主要方法:
- 微生物生产者的代谢工程,以增强目标活性和抑制不良途径.
- 使用全细胞催化和酶方法进行选择性类固醇修饰.
- 通过类固醇生成系统的异质表达来进行向氧化.
主要成果:
- 代谢工程能够增强所需类固醇前体的生产和新代谢物的积累.
- 酶的方法促进精确的结构修改的类固醇,包括氧化和还原.
- 类固醇生成系统的异质表达导致基化类固醇的选择性形成.
结论:
- 代谢工程和合成生物学是推进微生物类固醇生物技术的关键驱动力.
- 这些技术有助于开发用于类固醇合成的改进和新型微生物生物催化剂.
- 该领域在有效和可持续生产有价值的类固醇化合物方面显示出重大前景.
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