Search research articles

关于 JoVE

概览领导团队博客 JoVE 帮助中心

作者

出版流程编辑委员会范围与政策同行评审常见问题投稿

图书馆员

用户评价订阅访问资源图书馆顾问委员会常见问题

研究

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments 存档

教育

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual 教师资源中心教师网站

使用条款与条件

相关概念视频

Bioremediation

Bioremediation

Bioremediation is the use of prokaryotes, fungi, or plants to remove pollutants from the environment. This process has been used to remove harmful toxins in groundwater as a byproduct of agricultural run-off and also to clean up oil spills.

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序

Same author

Waterborne Protozoan Parasite and Thalassogenic Diseases in Marine Environment: Detection Techniques, Indicators and Public Health Implications.

Microorganisms·2026

Same author

Evidence of Waterborne Parasites in Mussels for Human Consumption Harvested from a Recreational and Highly Productive Bay.

Microorganisms·2025

Same author

A comprehensive review of treatment technologies for greywater contaminants: an emphasis on surfactants.

Journal of environmental management·2025

Same author

Green Synthesis of Fe<sub>2</sub>O<sub>3</sub> Nanoparticles Using <i>Eucalyptus globulus</i> Leaf Extract on <i>Pinus radiata</i> Sawdust for Cationic Dye Adsorption.

Nanomaterials (Basel, Switzerland)·2024

Same author

In Situ Synthesis of Cu<sub>2</sub>O Nanoparticles Using <i>Eucalyptus globulus</i> Extract to Remove a Dye via Advanced Oxidation.

Nanomaterials (Basel, Switzerland)·2024

Same author

Enhancing Water Status and Nutrient Uptake in Drought-Stressed Lettuce Plants (<i>Lactuca sativa</i> L.) via Inoculation with Different <i>Bacillus</i> spp. Isolated from the Atacama Desert.

Plants (Basel, Switzerland)·2024

Search research articles

相关实验视频

Updated: Jul 17, 2025

Isolation and Screening from Soil Biodiversity for Fungi Involved in the Degradation of Recalcitrant Materials

Isolation and Screening from Soil Biodiversity for Fungi Involved in the Degradation of Recalcitrant Materials

Published on: May 16, 2022

生物降解植物菌株的选择

María-Angélica Mondaca¹, Maricel Vidal¹, Soledad Chamorro^2,3

¹Microbiology Department, University of Concepción, Concepción, Chile.

Methods in molecular biology (Clifton, N.J.)

|August 29, 2023

概括

研究人员增强了植物生物转化细菌,Mycobacterium sp.,通过培养它们以高度的β-sitosterol. 这种选择方法显著提高了它们在将β-固醇转化为有价值的类固醇化合物的效率.

关键词:

生物转化生物转化自然产品是天然产品.类固醇是一种类固醇.β-固醇是Mycobacterium sp. 的一种细菌.

更多相关视频

Prospecting Microbial Strains for Bioremediation and Probiotics Development for Metaorganism Research and Preservation

Prospecting Microbial Strains for Bioremediation and Probiotics Development for Metaorganism Research and Preservation

Published on: October 31, 2019

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol

Published on: October 24, 2016

相关实验视频

Last Updated: Jul 17, 2025

Isolation and Screening from Soil Biodiversity for Fungi Involved in the Degradation of Recalcitrant Materials

Isolation and Screening from Soil Biodiversity for Fungi Involved in the Degradation of Recalcitrant Materials

Published on: May 16, 2022

Prospecting Microbial Strains for Bioremediation and Probiotics Development for Metaorganism Research and Preservation

Prospecting Microbial Strains for Bioremediation and Probiotics Development for Metaorganism Research and Preservation

Published on: October 31, 2019

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol

Published on: October 24, 2016

科学领域:

微生物学微生物学
生物技术是生物技术.
生物化学工程生物化学工程

背景情况:

植物醇,像β-醇,是植物衍生的化合物,具有潜在的工业应用.
植物醇的有效微生物生物转化成有价值的类固醇基对于它们的利用至关重要.
开发具有增强生物转化能力的强壮微生物菌株是一个正在进行的研究领域.

研究的目的:

选择和增强Mycobacterium sp.的植物生物转化菌株.
为了研究高β-sitosterol度作为提高菌株的选择性压力的影响.
确定关键的生物转化产品并评估所选菌株的提高效率.

主要方法:

在培养Mycobacterium sp. 在介质中使用14g/Lβ-sitosterol作为唯一碳来源的菌株.
在两个月内连续转移细菌培养以施加选择性压力.
使用甲醇和乙烯酸乙烯提取生物转化产品.
使用薄层染色学,气液染色学 (GLC) 和GLC质谱学进行定性和定量分析.

主要成果:

选择的菌株是Mycobacterium sp. 这种细菌. 在7次转移后,MB-3683和Mycobacterium fortuitum B-11045的生物转化能力增加.
MB-3683的生物转化效率从20%提高到64%,B-11045.5的生物转化效率从34%提高到55%.
安德罗斯坦迪和安德罗斯坦迪被确定为主要的生物转化产物.

结论:

高度的β-固醇作为一个有效的选择机制,用于隔离高效的植物生物转化菌株.
开发的方法显著增强了从β-sitosterol中微生物生产的类固醇基.
这种方法有望通过微生物生物转化在工业规模生产类固醇化合物.