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相关概念视频

RNA-seq03:21

RNA-seq

10.1K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.1K
Ribosome Profiling02:24

Ribosome Profiling

3.6K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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相关实验视频

Updated: Jul 17, 2025

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
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Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

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RNA制备和RNA-Seq生物信息学用于比较转录组学.

Antonio Rodríguez-García1,2, Alberto Sola-Landa3,4, Carlos Barreiro3,5

  • 1Área de Microbiología, Departamento de Biología Molecular, Facultad de Ciencias Biológicas y Ambientales, Universidad de León, León, Spain. arodg@unileon.es.

Methods in molecular biology (Clifton, N.J.)
|August 29, 2023
PubMed
概括

本研究详细介绍了RNA测序 (RNA-seq) 的RNA提取和生物信息学协议,以识别差异表达的基因. 它的目标是使这种强大的转录组分析能够在微生物学实验室中使用.

关键词:
生物导体是一种生物导体.不同表达式的差异表达式提取RNA 提取RNA的方法在RNA-seqqq.文字转录学 (Transcriptomics) 是一个学科.

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Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis
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Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis

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A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq
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A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq

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相关实验视频

Last Updated: Jul 17, 2025

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
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A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq
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A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq

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科学领域:

  • 分子生物学分子生物学
  • 生物信息学是一种生物信息学.
  • 基因组学就是基因组学.

背景情况:

  • 差异性基因表达分析在转录学学中至关重要.
  • 与RT-qPCR和微阵列相比,RNA测序 (RNA-seq) 提供了更高的准确性,动态范围和转录检测.
  • 许多微生物学实验室由于感知到的复杂性或成本而没有采用RNA-seq.

研究的目的:

  • 为RNA提取提供详细的协议.
  • 为RNA-seq数据引入生物信息学分析.
  • 为了使产生差异性基因表达结果.

主要方法:

  • 这是一个RNA提取协议.
  • 生物信息学管道用于RNA-seq数据分析.
  • 不同基因表达分析.

主要成果:

  • 介绍了RNA提取的综合性协议.
  • 为RNA-seq数据提供生物信息学分析的入门指南.
  • 该协议有助于识别差异表达的基因.

结论:

  • RNA-seq是微生物学中转录组分析的强大工具.
  • 该协议简化了RNA提取和生物信息学分析.
  • 鼓励在微生物学实验室中增加RNA-seq的采用.