扩大植物的生物转化成安德罗二的规模
Sonia Martínez-Cámara1, Manuel de la Torre1, José-Luis Barredo1
1Department of Biotechnology, Curia Spain, León, Spain.
Methods in molecular biology (Clifton, N.J.)
|August 29, 2023
概括
这项研究扩大了植物生物转化成androstenedione (AD) 的规模,使用大型生物反应器中的两相系统. 这种方法有效地将植物醇转化为有价值的类固醇中间体.
科学领域:
- 生物技术是生物技术.
- 微生物生物转化
- 类固醇的化学成分
背景情况:
- 来自植物油和木质纸的植物,具有类固醇核.
- 植物的生物转化可以通过切割C17侧链产生重要的类固醇中间体.
- 植物和产品的水溶性差,需要专门的生物处理技术.
研究的目的:
- 为了扩大植物的生物转化到4-androstene-3,17-dione (androstenedione; AD).
- 在生物反应器中使用Mycolicibacterium neoaurum B-3805来优化该过程.
- 用水-油两相系统解决溶解难题.
主要方法:
- 使用Mycolicibacterium neoaurum B-3805用于植物的生物转化.
- 采用水油双相系统来克服基板和产品的不溶性.
- 在5L和20L生物反应堆中进行了规模化实验.
主要成果:
- 实现了植物醇到androstenedione (AD) 的有效生物转化.
- 成功地将该过程从瓶式扩展到20升生物反应器.
- 证明了高达20g/L的植物的生物转化能力.
结论:
- 水油双相系统对于大规模的植物生物转化到AD.有效.
- Mycolicibacterium neoaurum B-3805 是一个适合这种工业生物转换的微生物.
- 这种可扩展的过程为从植物中生产类固醇中间体提供了可行的途径.
关键词:
4-安德罗-3,17-氨酸是一种AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD AD ADD was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was was安德罗斯二一个.生物转化生物转化发酵 发酵 是一个过程.这种细菌是Mycobacterium neoaurum.这种细菌是Mycolicibacterium neoaurum.植物醇是一种植物醇.两相系统是双相系统.相关概念视频
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