植物醇通过Mycolicibacterium生物转化为安德罗二
Kjell Domaas Josefsen1, Anna Nordborg1, Simone Balzer Le2
1Department of Biotechnology and Nanomedicine, SINTEF Industry, Trondheim, Norway.
Methods in molecular biology (Clifton, N.J.)
|August 29, 2023
概括
菌根菌的细菌将植物固醇转化为有价值的类固醇,如安德罗二 (AD). 这种生物转化过程使用环氧素优化,克服了水不溶性化合物用于制造类固醇的挑战.
科学领域:
- 生物技术是生物技术.
- 微生物化学 微生物化学
- 类固醇合成的方法
背景情况:
- 植物醇是植物油精炼和木制造的副产品,与类固醇具有共同的核心结构.
- 微生物生物转化提供了一种途径,通过修改它们的侧链,将植物转化为高价值的类固醇.
- 菌根菌种能够降解植物,突变体积累关键的类固醇中间体.
研究的目的:
- 用Mycolicibacterium spp.来描述植物的生物转化为androstenedione (AD) 的过程.
- 详细介绍LC-MS分析植物和类固醇产品的方法.
- 提出克服微生物类固醇生产挑战的策略,特别是水不可溶性和产品毒性.
主要方法:
- 在培养Mycolicibacterium spp.的过程中. 在使用定义介质的震动瓶和发酵器中.
- 化学修饰的环素的应用,以提高植物和类固醇产品的可溶性.
- 液体染色学-质谱学 (LC-MS) 用于基质和产品的量化.
主要成果:
- 由Mycolicibacterium spp.成功地将植物生物转化为安德罗二 (AD) 和安德罗二 (ADD).
- 通过使用环氧化来有效管理水不溶性问题.
- 建立了LC-MS协议,用于准确分析生物转化过程.
结论:
- 使用Mycolicibacterium spp.进行微生物转化. 是从植物中生产有价值的类固醇的可行途径.
- 循环二烯的使用有效地解决了生物转化过程中的溶解性和潜在毒性挑战.
- 描述的方法为优化从植物中产生的工业规模类固醇生产提供了基础.
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