设计一个Streptococcus Cas9正义分子与RxQ PAM结合动机,用于在细菌中控制无PAM基因

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Updated: Jul 17, 2025

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
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Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

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Yuxi Teng1, Jian Wang1, Tian Jiang1

  • 1School of Chemical, Materials and Biomedical Engineering, College of Engineering, The University of Georgia, Athens, Georgia 30602, United States.

ACS synthetic biology
|August 29, 2023
PubMed
概括

研究人员发现了一种新的Cas9酶SeHCas9,具有放松的原体空间器相邻动机 (PAM) 要求. 工程变异提供了无PAM的基因编辑,扩大了细菌基因控制的可能性.

关键词:
克里斯普尔-Cas9是什么意思克里斯普里是什么?克里斯普里是什么?在Cas9工程方面.没有PAM的目标定位.基因抑制 基因抑制

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