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Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
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The instrumentation of atomic emission spectrometry (AES) involves various components, including atomization devices that convert samples into gas-phase atoms and ions. There are two main types of atomization devices: continuous and discrete atomizers.  Continuous atomizers, like plasmas and flames, introduce samples in a constant stream, while discrete atomizers inject individual samples using syringes or autosamplers. The most common discrete atomizer is the electrothermal atomizer.
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测量:多重排气呼吸凝聚物 - - 扫描使用快速电分析.

Ashlesha Bhide1, Mohammed A Eldeeb1, Madhavi Pali1

  • 1Department of Bioengineering, The University of Texas at Dallas, Richardson, Texas 75080, United States.

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概括

一个新的电化学传感器,READ 2.0,可非侵入性地检测出口气凝液 (EBC) 中的炎症生物标志物,用于呼吸系统疾病监测. 该平台为预测疾病严重程度和生存率的入侵方法提供了一个有希望的替代方案.

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科学领域:

  • 生物医学工程 生物医学工程
  • 呼吸系统医学 呼吸系统医学
  • 分析化学 分析化学

背景情况:

  • 呼出的呼吸凝聚物 (EBC) 是检测呼吸系统疾病生物标志物的非侵入性来源.
  • 目前用于呼吸道炎症的诊断方法具有侵入性,并且对监测具有挑战性.
  • 像IL-6,IL-1β,IL-8和hs-CRP这样的细胞因子生物标志物是呼吸系统疾病的关键指标.

研究的目的:

  • 开发和验证一个新的16-plexed电化学平台,READ 2.0,用于在EBC中检测多重生物标志物.
  • 评估READ 2.0传感器对关键炎症生物标志物的性能特征.
  • 评估基于EBC的生物标志物监测对预测呼吸系统疾病严重程度和结果的潜力.

主要方法:

  • 使用16个复合体的电化学传感器 (READ 2.0) 来同时检测EBC中的IL-6,IL-1β,IL-8和hs-CRP.
  • 确定传感器动态范围,检测极限和精度.
  • 对ELISA进行分析验证,评估相关性和偏差,并评估传感器的精度和稳定性.

主要成果:

  • 该READ 2.0平台展示了灵敏的检测,动态范围高达243 pg/mL和低检测极限 (IL-6/IL-1β的1 pg/mL,IL-8/hs-CRP的3 pg/mL).
  • 与ELISA相比,检测准确度在约85%至100%之间,相关性高 (R2>0.96) 和偏差低.
  • 传感器表现出极好的精度 (%CV < 7%) 和稳定性 (在6小时内变化< 10%).

结论:

  • 该READ 2.0电化学平台能够可靠,非侵入性多重检测EBC中的关键炎症生物标志物.
  • 这项技术在预测呼吸系统疾病严重程度和生存率方面显著有前途.
  • 使用READ 2.0监测EBC生物标志物可以作为管理呼吸系统疾病的重要参考点.