基于外膜囊泡的纳米混合体向与瘤相关的巨细胞,以增强训练的与免疫相关的疫苗生成的抗瘤活性
Jie Liang1,2, Fei Zhu1, Keman Cheng1
1CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China, 11 Beiyitiao, Zhongguancun, Beijing, 100190, China.
Advanced materials (Deerfield Beach, Fla.)
|August 29, 2023
概括
这项研究开发了新的细菌外膜囊泡纳米混合体,作为训练有素的免疫相关疫苗 (TIrV),以增强抗瘤活性. 这种TIrV有效地训练免疫细胞,并触发瘤细胞细胞分裂,提供了一个新的疫苗接种策略.
科学领域:
- 免疫学 免疫学 免疫学
- 疫苗学 疫苗学 疫苗学
- 纳米技术纳米技术
背景情况:
- 训练有素的免疫包括对感染和疫苗接种的天生的免疫记忆.
- 细菌外膜囊泡 (OMV) 显示出作为训练有素免疫相关疫苗 (TIrV) 的潜力,但面临着交付和有效性挑战.
- 与瘤相关的巨细胞 (TAMs) 在抗瘤反应中起着至关重要的作用.
研究的目的:
- 开发和描述基于OMV的纳米混合体作为TIrV用于增强抗瘤活性.
- 调查训练免疫诱导和TAM介导的细胞分裂的机制.
- 为了阐明TIrV介导的抗瘤作用的代谢和表观遗传特征.
主要方法:
- 开发OMV纳米混合体 (OMV-SIRPα@CaP/GM-CSF) 用于训练免疫诱导.
- 使用ATAC和转录组测序对代谢和表观遗传重新连接的分析.
- 在MC38和B16-F10瘤模型中评估TIrV疗效.
主要成果:
- 开发的OMV纳米混合体成功训练了免疫细胞并增强了抗瘤活性.
- 在TAM热瘤中,TIrV诱导训练免疫和激活T细胞反应,在TAM冷瘤中主要训练免疫.
- 代谢和表观遗传分析确定了TIrV介导的抗瘤机制的关键特征.
结论:
- 基于OMV的纳米混合体代表了训练有素免疫相关疫苗的有希望的平台.
- 该研究阐明了TIrV在不同瘤微环境中的多方面的机制.
- 这项研究为开发针对癌症的先进疫苗接种策略提供了基础.
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