一个环节性基因网络编程了C. elegans发育过程中异常miRNA转录的时间和剂量
Brian Kinney1, Shubham Sahu2, Natalia Stec1
1Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.
Developmental cell
|August 29, 2023
概括
两个核激素受体 (NHRs) 在C. elegans发育中控制时间基因表达. 这些与昼夜节律相关的NHR确保了稳健的发育过渡的精确时间和水平.
科学领域:
- 发展生物学 发展生物学
- 分子遗传学 分子遗传学
- 时间生物学 时间生物学
背景情况:
- 精确控制基因表达时间和水平对于发育至关重要.
- 通过这种机制,cis-和trans-acting因素同时调节时间和水平的机制尚未完全理解.
- 核激素受体 (NHRs) 是基因表达的关键调节者.
研究的目的:
- 为了阐明微RNA (miRNA) lin-4的转录脉冲在C. elegans发育过程中是如何调节的.
- 研究核激素受体 (NHRs) 在控制发育时间和基因表达剂量的作用.
- 探索日间时钟组件在时间模式中的进化重新布线.
主要方法:
- 确定了两个NHRs,NHR-85和NHR-23,作为C. elegans中lin-4转录的调节者.
- 研究了NHR-85/NHR-23异构体与lin-4调控元素的合作结合.
- 分析了分阶段NHR表达和LIN-42 (一个周期正义) 对脉冲时间,振幅和持续时间的影响.
主要成果:
- 每个幼虫阶段,NHR-85和NHR-23都会诱导一次lin-4表达脉冲.
- 参与昼夜节律的哺乳动物正经体Rev-Erb和ROR具有类似的功能.
- 林-42抑制NHR-85,有助于精确的时间和林-4转录的剂量控制.
结论:
- NHR-85和NHR-23作为异构体起作用,产生精确的lin-4的转录脉冲.
- 循环时钟组件在线虫中经历了进化性的重新使用,以将基因表达时间与转录剂量联系起来.
- 这项研究揭示了一种通过暂时控制基因表达来协调发育过渡的新机制.
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