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通过调节miR-93-5p/TXNIP轴,circRNA-SCAF8促进了血管内皮细胞的灭

Bing Wang¹, Xinyu Yu², Tianchi Chen³

¹Department of Vascular Surgery, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China. 3170104484@zju.edu.cn.

Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences

|August 29, 2023

概括

高葡萄糖通过circRNA-SCAF8/miR-93-5p/TXNIP通路诱导HUVEC中的热. 抑制circRNA-SCAF8或过度表达miR-93-5p可以减少热和相关因素,从而提供治疗点.

科学领域:

分子生物学分子生物学
细胞生物学细胞生物学
生物化学生物化学

背景情况:

高葡萄糖的环境可以诱导人静脉内皮细胞 (HUVECs) 的热亡.
了解调节内皮质细胞灭的分子机制对于管理高葡萄糖相关并发症至关重要.
循环RNAs (circRNAs) 已经成为各种细胞过程中的关键调节者,包括炎症和细胞死亡.

研究的目的:

为了研究circRNA-SCAF8在高葡萄糖条件下调节内皮细胞烧亡中的作用.
为了阐明涉及circRNA-SCAF8/miR-93-5p/TXNIP轴的潜在分子机制.
评估针对治疗干预这一轴的潜在目标.

主要方法:

培养HUVEC并使用不同度的葡萄糖和特定的抑制剂/过度表达分子进行处理.
用CCK-8试验,流细胞计和光染色来评估细胞活力和热.
烧灭相关因子 (例如NLRP-3,caspase-1,GSDMD,TXNIP) 和circRNA-SCAF8/miR-93-5p的表达水平使用西式涂抹,ELISA和qRT-PCR进行了量化.
采用双 luciferase 试验和光 in situ 杂交 (FISH) 来确认分子相互作用和局部化.

主要成果:

抑制circRNA-SCAF8或miR-93-5p的过度表达显著增加了HUVEC活力和降低了烧灭.
这些干预措施还导致了与热致死相关因素的显著减少,包括TXNIP,NLRP-3,caspase-1,GSDMD,IL-18和IL-1β.
双 luciferase 试验证实,miR-93-5p 直接针对 circRNA-SCAF8 和 TXNIP,建立了 circRNA-SCAF8/miR-93-5p/TXNIP 调节轴.

结论:

在HUVEC中,circRNA-SCAF8/miR-93-5p/TXNIP轴在调节高葡萄糖诱导的热中发挥着至关重要的作用.
环形RNA-SCAF8促进热,而miR-93-5p通过准环形RNA-SCAF8和TXNIP.p作为抑制剂.
这一途径代表了治疗糖尿病血管并发症的潜在治疗目标.

关键词:

循环RNA-SR相关的CTD相关因子糖尿病是2型糖尿病.人的带静脉内皮细胞在米R-93-5p.热性灭症 (Pyroptosis) 是一种铁素相互作用的蛋白质.

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