相关实验视频

Updated: Jul 17, 2025

Technical Applications of Microelectrode Array and Patch Clamp Recordings on Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Technical Applications of Microelectrode Array and Patch Clamp Recordings on Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Published on: August 4, 2022

基于转录基因组的药物预测,抑制人类诱导多能干细胞中的心肌发生

Anna Cherianidou¹, Franziska Kappenberg², Florian Seidel³

¹University of Cologne, Faculty of Medicine and University Hospital Cologne, Center for Physiology, Working Group Sachinidis, 50931 Cologne, Germany Robert-Koch-Str. 39, 50931, Cologne, Germany.

Cell death discovery

|August 29, 2023

概括

人类诱导的多能干细胞 (hiPSCs) 为研究发育性心脏毒性提供了一个相关的模型. 研究人员确定了一种基因特征和一个新的索引,以预测化合物如何影响心脏发育,改善安全评估.

科学领域:

发展性毒理学发展性毒理学
干细胞生物学干细胞生物学
心血管研究的心血管研究.

背景情况:

用于胚胎毒性测试的动物模型受到成本,复杂性和人类相关性的限制.
从动物研究中预测人类发展结果时,物种差异带来了挑战.
人类诱导的多能干细胞 (hiPSCs) 为人类发育毒理学提供了一个有希望的体外模型.

研究的目的:

建立一个与人类相关的体外模型,用于评估心肌生成过程中的心脏毒性.
识别一种基因特征,表明心脏发育受损.
开发环境因素和治疗药物的心脏毒性预测指数.

主要方法:

调节Wnt信号通路以复制hiPSC中的心肌生成.
对已知原体和非原体的hiPSC暴露.
一个31基因早期心肌形成特征的识别.
开发和验证"发育性心脏毒性指数" (CDI31g).

主要成果:

包括13-cis-酸在内的视网类药物被确定为hiPSC心肌生成的强有力的抑制剂.
鉴定出一种特定的31基因特征和受影响的生物过程是对视网类药物的反应.
在CDI31g准确地区分了原体与非原体,关于它们对hiPSC差异化的影响.
该研究使用hiPSC模型成功识别了损害心脏发育的化合物.

结论:

hiPSC为评估人类发育性心脏毒性提供了一个有价值的平台.
鉴定的基因特征和CDI31g为预测心脏毒性影响提供了一种新的方法.
该模型系统增强了对潜在治疗药物和环境剂的安全性评估.
这些发现有助于减少对胚胎毒性评估的动物试验的依赖.

更多相关视频

Contractions of Human-iPSC-derived Cardiomyocyte Syncytia Measured with a Ca-sensitive Fluorescent Dye in Temperature-controlled 384-well Plates

Contractions of Human-iPSC-derived Cardiomyocyte Syncytia Measured with a Ca-sensitive Fluorescent Dye in Temperature-controlled 384-well Plates

Published on: October 18, 2018

High-Throughput Cardiotoxicity Screening Using Mature Human Induced Pluripotent Stem Cell-Derived Cardiomyocyte Monolayers

High-Throughput Cardiotoxicity Screening Using Mature Human Induced Pluripotent Stem Cell-Derived Cardiomyocyte Monolayers

Published on: March 24, 2023

相关概念视频

EPS and iPS Cells in Disease Research

EPS and iPS Cells in Disease Research

Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...