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Updated: Jul 17, 2025

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
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Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

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深度和快速的无标签的动态器官细胞映射.

Julia P Schessner1, Vincent Albrecht1, Alexandra K Davies1,2

  • 1Department of Proteomics and Signal Transduction, Systems Biology of Membrane Trafficking Research Group, Max-Planck Institute of Biochemistry, Martinsried, Germany.

Nature communications
|August 29, 2023
PubMed
概括

我们使用数据独立获取 (DIA) -DOMs增强了蛋白质定位映射,实现了更大的深度和精度. 这种改进的空间蛋白质组学工作流程有助于发现细胞表型和理解蛋白质动态.

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Protein Dynamics in Living Cells01:19

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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