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Updated: Jul 17, 2025

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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提高量化准确度在异标记质谱实验与光谱图书馆搜索和基于特征的-光谱匹配过器的质谱测试实验

Tzu-Yun Kuo1, Jen-Hung Wang2,3,4, Yung-Wen Huang5

  • 1Department of Biochemical Science and Technology, National Taiwan University, Taipei, 10617, Taiwan.

Scientific reports
|August 29, 2023
PubMed
概括

这项研究引入了一种新的蛋白质量定量管道,用于同位素标记质谱. 它通过将数据库和光谱库搜索与基于新型特征的-光谱匹配过器相结合来提高准确性.

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Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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