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相关概念视频

Transmission Electron Microscopy01:15

Transmission Electron Microscopy

5.6K
In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400...
5.6K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
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Overview of Electron Microscopy01:25

Overview of Electron Microscopy

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The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
9.2K
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

4.3K
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
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Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

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The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
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Cryo-electron Microscopy01:28

Cryo-electron Microscopy

3.4K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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相关实验视频

Updated: Jul 17, 2025

Author Spotlight: A Machine-Vision Approach to Transmission Electron Microscopy Workflows, Results Analysis and Data Management
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Author Spotlight: A Machine-Vision Approach to Transmission Electron Microscopy Workflows, Results Analysis and Data Management

Published on: June 23, 2023

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该abTEM代码:从第一原则传输电子显微镜的第一原则.

Jacob Madsen1, Toma Susi1

  • 1Faculty of Physics, University of Vienna, Vienna, 1090, Austria.

Open research Europe
|August 30, 2023
PubMed
概括
此摘要是机器生成的。

我们开发了abTEM,这是一个开源的Python代码,用于模拟传输电子显微镜 (TEM) 图像. 它集成了密度函数理论 (DFT) 来捕捉价值键效应,使实验数据的分析更准确.

关键词:
在这里,Python是Python.密度函数理论密度函数理论电子散射是一种电子散射.图像模拟图像的模拟分子动力学分子动力学这是开源的,开源的.传输电子显微镜的使用

更多相关视频

Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography
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Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography

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Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy
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Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy

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相关实验视频

Last Updated: Jul 17, 2025

Author Spotlight: A Machine-Vision Approach to Transmission Electron Microscopy Workflows, Results Analysis and Data Management
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Author Spotlight: A Machine-Vision Approach to Transmission Electron Microscopy Workflows, Results Analysis and Data Management

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Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography
08:04

Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography

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Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy
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Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy

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科学领域:

  • 材料科学 材料科学 材料科学
  • 计算物理 计算物理
  • 化学 化学 化学

背景情况:

  • 传输电子显微镜 (TEM) 模拟对于解释实验数据至关重要.
  • 通常使用的独立原子模型忽略了价值键,限制了准确性.
  • 先进的仪器仪表揭示了以前无法观察到的微妙的散射潜力的细节.

研究的目的:

  • 开发一个开源的模拟代码,abTEM,用于先进的TEM图像和衍射模式分析.
  • 整合密度函数理论 (DFT) 的计算,以准确地确定散射电位.
  • 为研究人员提供一个用户友好,模块化和可扩展的基于Python的工具.

主要方法:

  • 开发了abTEM,这是一个基于Python的模拟代码,集成DFT用于散射潜力计算.
  • 实施了模块化软件设计,以实现灵活性和可扩展性.
  • 利用开源库,如原子仿真环境和GPAW,以提高性能和功能.
  • 为交互式Python笔记本设计,提供从结构定义到结果分析的无工作流.

主要成果:

  • 证明了abTEM在六角化中检测价值键的能力.
  • 执行了4D-STEM模拟二硫化物与图形相重建的模拟.
  • 用聚变束电子衍射对系统进行分子动力学 (MD) 和冷声子建模的比较.
  • 对金纳米粒子PRISM算法实现的性能进行了评估.

结论:

  • abTEM提供了一个强大的,可访问的工具,用于先进的TEM模拟,并结合了价值结合效应.
  • 该代码与DFT和模块化设计的集成有助于准确和可重复的材料分析.
  • abTEM支持各种成像模式和算法开发,增强其对研究人员的实用性.