一个新的命名为与畜牧业相关的结核菌菌综合体,基于家族遗传学
Michaela Zwyer1,2, Cengiz Çavusoglu3, Giovanni Ghielmetti4
1University of Basel, Basel, Switzerland.
Open research Europe
|August 30, 2023
概括
一个新的命名法分类了与牲畜相关的Mycobacterium结核病综合体 (MTBC) 细菌,包括Mycobacterium bovis,M. caprae和M. orygis. 这种基于全基因组测序的系统识别了三个主要血统和八个子血统,改善了结核病研究.
科学领域:
- 基因组学和植物遗传学
- 兽医微生物学 兽医微生物学
- 流行病学 流行病学
背景情况:
- 结核菌复合体 (MTBC) 在人类和动物中引起结核病 (TB).
- 现有的命名体系有效地追踪了适应人类的MTBC菌株,但缺乏牲畜相关菌株 (Mycobacterium bovis,M. caprae,M. orygis) 的细节.
- 新兴的基因组学研究揭示了牲畜相关的MTBC的显著遗传多样性,需要更新的分类系统.
研究的目的:
- 为畜牧业相关的MTBC成员 (M. bovis,M. caprae,M. orygis) 建立一个全面的命名体系.
- 基于全基因组测序数据,对这些细菌群体内的遗传多样性进行分类.
- 促进动物结核病的分子流行病学和进化研究.
主要方法:
- 来自公共数据库的8736个全基因组序列 (WGS) 的分析,以及39个新测序的菌株.
- 选择829个WGS的代表子集,以捕捉全球遗传多样性.
- 植物遗传学和遗传多样性分析的应用,以定义细菌群体和亚系.
主要成果:
- 关于M. bovis,M. caprae和M. orygis. 的三个主要遗传系 (La1,La2,La3) 的建议.
- 在La1谱系 (La1.1-La1.8) 中识别了八个不同的亚系,地理分布不同.
- 开发用于基因定型的基因组学信息单核酸多态 (SNP) 标记物,集成到开源工具中 (KvarQ,TB-Profiler).
结论:
- 拟议的分类法增强了畜牧业相关的MTBC中遗传多样性的分类.
- 这种改进的分类将支持未来对动物群体结核病的分子流行病学和进化研究.
- 提供SNP条形码和更新的工具将有助于在社区范围内对MTBC菌株进行分子表征.
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