乙型肝炎病毒血清RNA转录异形的组成和比例在慢性乙型肝炎患者通过纳米孔长读测序
Alicia Vachon1,2, Grace E Seo1,2, Nishi H Patel3
1Department of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, MB, Canada.
Frontiers in microbiology
|August 30, 2023
概括
血清乙型肝炎病毒 (HBV) RNA是慢性乙型肝炎 (CHB) 的关键生物标志物. 这项研究使用纳米孔测序对血清中的HBV RNA转录进行了表征,揭示了基因前RNA (pgRNA) 作为主导形式,并确定了新的拼接变异.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 生物标志物发现发现
背景情况:
- 血清乙型肝炎病毒 (HBV) RNA是管理和预测慢性乙型肝炎 (CHB) 感染结果的新兴生物标志物.
- 在封闭的颗粒中,HBV血清转录组的精确组成,目标分析物,还不清楚.
- 鉴定血清HBVRNA对于改善CHB诊断和治疗监测至关重要.
研究的目的:
- 评估血清HBVRNA的转录组成和比例性.
- 分析不同HBV基因型 (A-F) 和HBeAg状态的这些特征.
- 评估PCR-cDNA纳米孔测序对血清HBVRNA分析的有用性.
主要方法:
- 从13名CHB患者的血清样本使用PCR-cDNA纳米孔测序分析.
- 三个生物信息工作流程 (FLAIR,RATTLE,GraphMap) 用于HBV RNA异型的检测和量化.
- 一种尖端RNA变异 (SIRV) 控制混合物被用于验证运行质量和覆盖范围.
主要成果:
- 在生物信息学工作流中,观察到关于转录比例和组成的高度一致性.
- 肝炎病毒前基因组RNA (pgRNA) 是最丰富的转录 (93.8%的样本),其他变体包括拼接和3'截断形式.
- 剪接的pgRNA变异,特别是Sp1,在基因型B,C,E和F中更常见;纵向数据显示,在接受治疗的个体中,妊娠/产后期间,3'截断变异的增加.
结论:
- 长读测序是鉴定血清HBV转录基因的一个有价值的工具.
- 需要进行进一步的研究,以将特定的血清HBVRNA异型及其比例与CHB疾病进展和治疗反应相关联.
- 了解血清HBVRNA概况可以提高CHB的临床管理策略.
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