在社会虫中对G蛋白结合受体进行全系分析,以及对多细胞性进化的含义
Grant Hall1, Sarah Kelly1,2, Pauline Schaap1
1School of Life Sciences, University of Dundee, Dundee, DD1 5EH, UK.
Open research Europe
|August 30, 2023
概括
G蛋白结合受体 (GPCRs) 对于Amoebozoa的感知至关重要. 这项研究揭示了GPCRs在dictyostelids中进化,可能使多细胞发育和通信.
科学领域:
- * 分子生物学 * 分子生物学
- * 进化生物学 进化生物学
- * 基因组学 是一个学科.
背景情况:
- *G蛋白结合受体 (GPCRs) 是一个大家族的真核细胞跨膜蛋白.
- *它们在单细胞阿米波动物中的存在表明它们具有复杂的环境传感能力.
- *在进化过渡到多细胞化过程中,GPCRs可能被重新定位为细胞-细胞通信.
研究的目的:
- *分析Amoebozoa中GPCRs的遗传分布,域组成和表达模式.
- *研究GPCRs的进化史及其在多细胞发育中的潜在作用.
- * 识别可能在社会虫中被选择用于信号的新型GPCRs.
主要方法:
- *从测序的阿米波动物基因组中对GPCR进行贝叶斯推理分析.
- * 遗传学分布和域组成的比较.
- *对五种dityostelid物种进行时间和空间表达模式分析.
主要成果:
- * 大多数GPCR在阿米波动物中深度保存,参与细胞功能和环境传感.
- *除了真菌激素受体外,所有GPCR家族都存在于dictyostelids中.
- * 第1个家族的罗多类GPCR首次在dictyostelids中被发现.
- * 一些GPCR被放大并可能重新用于多细胞发育信号.
结论:
- *GPCR家族1,2和6在阿米波动物中早期分离;家族3和5在后来扩大.
- *家族6的cAMP受体,对于dictyostelid发育至关重要,起源于早期.
- * 候选GPCRs在dictyostelids中进化,可能被选择用于多细胞发育.
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