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相关概念视频

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
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相关实验视频

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Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy
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Qudi-HiM:一个开源的采集软件包,用于高度多重的序列和组合光学成像.

Franziska Barho1, Jean-Bernard Fiche1, Marion Bardou1

  • 1Centre de Biologie Structurale, Centre National de la Recherche Scientifique, UMR5048, Montpellier, 34090, France.

Open research Europe
|August 30, 2023
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概括

我们开发了Qudi-HiM,这是一个Python软件包,用于自动化用于空间多omics的多重成像. 这种工具增强了在定制显微镜上研究单细胞生物学和染色质组织的研究.

关键词:
染色体组织是染色体组织.多重成像多重成像成像光学显微镜的光学显微镜.转录 转录 是一种转录.

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科学领域:

  • 细胞和分子成像技术
  • 生物技术是生物技术.
  • 生物信息学是一种生物信息学.

背景情况:

  • 多复合成像允许在单细胞分辨率下同时检测多个生物分子 (蛋白质,DNA,RNA) 的空间多基因组.
  • 最近开发了Hi-M (高含量成像 - 多重复合) 方法来研究单细胞内的染色质空间组织.

研究的目的:

  • 开发一个模块化软件包,Qudi-HiM,在定制显微镜设置上实现Hi-M序列成像.
  • 为了自动获取和分析复杂的成像数据,用于空间多omics研究.

主要方法:

  • 开发Qudi-HiM,这是一个Python 3软件包,包含用于自动图像采集,微流体处理和实时监控的模块.
  • 实施强大的获取数千个3D多色显微镜图像的实施.
  • 集成远程监控和实时分析能力.

主要成果:

  • Qudi-HiM成功地在定制显微镜上为Hi-M自动化多重复序列成像.
  • 该软件有助于处理微流体设备,并提供实时数据分析.
  • Qudi-HiM非常通用,可以作为各种成像模式的独立工具来使用.

结论:

  • Qudi-HiM提供了一个强大而灵活的软件解决方案,用于先进的空间多omics研究.
  • 开发的工具提高了高分辨率蜂成像的可访问性和效率.
  • Qudi-HiM支持更广泛的科学界推进单细胞空间生物学研究.