针对SARS-CoV-2变种的宽谱人性化单克隆中和抗体,包括Omicron变种
Kun Wen1, Jian-Piao Cai2, Xiaodi Fan1,3
1Microbiome Medicine Center, Division of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
Frontiers in cellular and infection microbiology
|August 30, 2023
概括
研究人员开发出强大的单克隆抗体 (mAbs),有效对抗SARS-CoV-2变种,包括Omicron. 这些抗体减少了仓鼠的病毒载量,为未来的COVID-19治疗提供了有希望的策略.
科学领域:
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
- 药物发现 药物发现 药物发现
背景情况:
- 针对SARS-CoV-2尖端蛋白的治疗单克隆抗体 (mAbs) 对于治疗严重的COVID-19至关重要.
- 新兴的SARS-CoV-2变种具有尖端蛋白突变,可以逃避现有的mAb疗法,需要新的治疗策略.
研究的目的:
- 产生和表征具有广泛中和活性的新型单克隆抗体 (mAbs) 针对SARS-CoV-2变种.
- 在临床前模型中评估mAb候选物的体内疗效.
主要方法:
- 采用混合体技术,从用SARS-CoV-2尖端蛋白和受体结合域 (RBD) 免疫的小鼠中产生mAbs.
- 一组mAbs被选为对抗各种SARS-CoV-2变异的结合和中和活性.
- 领先的mAb,WKS13的体内有效性在SARS-CoV-2感染的仓鼠模型中进行了评估.
主要成果:
- 确定了18个结合尖端蛋白的mAbs,其中10个针对RBD.
- 五个针对RBD的mAbs中和了祖先的SARS-CoV-2菌株,并阻止了ACE2结合.
- 一个mAb,WKS13,对包括Omicron在内的所有令人担忧的变种 (VOC) 进行了广泛的中和,并在感染Delta的仓鼠中减少了肺病毒载量.
结论:
- 可以使用免疫小鼠生成宽频谱,高强度的mAbs,然后将其人性化用于治疗用途.
- 描述的方法为开发针对新出现的SARS-CoV-2变种的治疗mAbs提供了快速和多功能方法.
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