由加快力显示的α-catenin依赖的E-cadherin粘附动力学
Joshua Bush1,2, Jolene I Cabe3, Daniel Conway4
1Mechanical & Aerospace Engineering, Old Dominion University, Norfolk, VA 23529 USA.
bioRxiv : the preprint server for biology
|August 30, 2023
概括
埃卡德林的粘附强度和移动性直接相关,而α-catenin对于维持细胞-细胞接触稳定性至关重要. 这项研究揭示了这些动态如何调节组织重塑.
科学领域:
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
- 生物化学 生物化学
背景情况:
- 细胞与细胞的接触动态对于组织重塑和形状变化至关重要.
- 表皮细胞形状是由E-cadherin粘附动力学调节的,受力的影响.
- 之前,E-cadherin粘附强度和移动性之间的关系尚不清楚.
研究的目的:
- 为了研究E-cadherin粘附强度和依赖力运动之间的关系.
- 探索α-catenin在E-cadherin粘附动态中的作用.
主要方法:
- 利用磁拉动细胞测量来对磁性微珠和上皮细胞之间的E-cadherin粘合物施加力坡道.
- 测量了个体E-cadherin粘附强度和依赖强力的运动性.
- 检查了带有和没有α-catenin的上皮细胞中的E-cadherin粘附动力学.
主要成果:
- 发现了E-cadherin粘附强度和依赖力移动性之间的直接相关性.
- 缺乏α-catenin的上皮细胞表现出降低了E-cadherin的粘附强度和增加了移动性.
- α-catenin对于恢复应变的细胞与细胞接触至关重要.
结论:
- 粘附强度和E-cadherin粘附的取决于力量的移动性共同工作,以保持细胞与细胞接触恒温.
- 在调节E-cadherin粘附动态方面,α-catenin起着关键的形态作用.
- 这项研究提出了一种新的方法,用于将力依赖的粘附流动性与粘附强度联系起来.
相关概念视频
Tension Response at Adherens Junctions
2.7K
The adherens junctions that anchor cells together are multi-protein complexes that dynamically adapt to mechanical stimuli such as tensile forces and shear stress. Mechanosensory proteins in these junctions can sense such mechanical stimuli and undergo a shift in their conformation, resulting in an altered function — a process called mechanotransduction.
α-Catenin as a Mechanosensory Protein
The α-catenin of adherens junctions is an allosteric protein with three VH (vinculin...
α-Catenin as a Mechanosensory Protein
The α-catenin of adherens junctions is an allosteric protein with three VH (vinculin...
2.7K
Catenins
2.3K
Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the...
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the...
2.3K
Intracellular Signaling Affects Focal Adhesions
2.7K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Some...
2.7K
Adherens Junctions
4.9K
Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types – adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic
Adherens Junctions are Dynamic
4.9K
Cell-matrix's Response to Mechanical Forces
2.7K
In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue.
Anchoring junctions mechanically attach a cell to the...
Anchoring junctions mechanically attach a cell to the...
2.7K
Mechanism of Lamellipodia Formation
2.6K
Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
2.6K


