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RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

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Updated: Jul 17, 2025

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

一个基于工程设计的,双组件导向RNA的分子近距离传感器.

Junhong Choi^1,2, Wei Chen^1,3, Hanna Liao^1,4,5

¹Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA.

bioRxiv : the preprint server for biology

|August 30, 2023

概括

科学家们开发了"P3编辑",一种新的方法,将蛋白质相互作用与CRISPR-Cas9基因组编辑联系起来. 这允许精确的基因编辑在细胞中,由分子信号控制,用于先进的合成生物学应用.

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Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines

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Published on: June 2, 2018

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Last Updated: Jul 17, 2025

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

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科学领域:

合成生物学合成生物学
分子生物学分子生物学
基因组工程是基因组工程.

背景情况:

合成生物学旨在在活细胞中创建可编程的分子电路.
像CRISPR-Cas9这样的基因组编辑工具对于这些电路至关重要,但编程它们的激活是具有挑战性的.
通过蛋白质相互作用等分子事件控制基因组编辑是一个关键的未满足需求.

研究的目的:

开发一种用于使用蛋白质-蛋白质相互作用编程CRISPR-Cas9基因组编辑的新策略.
通过工程近距离依赖导向RNA来证明主要编辑和基编辑的激活.
为了探索RNA感应基因组编辑的RNA感应途径的整合.

主要方法:

为CRISPR-Cas9系统设计了一种双元导向RNA.
链接的蛋白质-蛋白质相互作用和化学诱导的二分化以引导RNA的形成.
在人类细胞中测试了P3编辑策略,用于主要编辑和基础编辑.
集成的基于ADAR的RNA传感器来触发基因组编辑.

主要成果:

证明蛋白质的接近可以激活CRISPR-Cas9主要编辑和基编辑.
展示了各种已知的蛋白质-蛋白质相互作用和化学诱导剂的使用.
成功将RNA传感途径与特定的基因组编辑事件联系起来.
在合成电路中增强了基于CRISPR的基因组编辑的可控性.

结论:

P3编辑提供了一个强大的方法来控制基因组编辑与分子输入.
这一战略显著推动了复杂合成分子电路的开发.
能够在活细胞内进行精确和可编程的基因组修改,用于各种应用.