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相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

57.4K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
57.4K

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相关实验视频

Updated: Jul 17, 2025

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
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High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

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使用CRISPR-Cas13进行高度多重化的mRNA量化.

Brian Kang1, Jiayu Zhang1, Michael P Schwoerer1

  • 1Department of Molecular Biology, Princeton University, Princeton, NJ 08544.

bioRxiv : the preprint server for biology
|August 30, 2023
PubMed
概括

核酸多重评估的定量组合阵列反应 (qCARMEN) 能够实现高通量,具有成本效益的RNA量化. 这种方法分析了超过4,500个基因样本对,推进了多基因研究.

科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 生物技术是生物技术.

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Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines
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A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
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相关实验视频

Last Updated: Jul 17, 2025

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
07:27

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

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Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines
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背景情况:

  • 现有的RNA量化方法在高吞吐量和成本效益之间存在权衡.
  • 剖析转录组通常是昂贵的,而低成本方法的范围是有限的.

结论:

  • qCARMEN提供了一个简单,廉价和可扩展的RNA量化解决方案.
  • 提高了多基因运动研究的可行性,基因调控网络分析和组合基因选.
  • 扩展了转录学中的应用,具有高吞吐量和成本效益.