相关实验视频

Updated: Jul 17, 2025

Live Imaging Assay for Assessing the Roles of Ca2+ and Sphingomyelinase in the Repair of Pore-forming Toxin Wounds
18:25

Live Imaging Assay for Assessing the Roles of Ca2+ and Sphingomyelinase in the Repair of Pore-forming Toxin Wounds

Published on: August 25, 2013

11.7K

膜结合和孔隙形成是Ca2+-依赖于Clostridioides difficile的二进制毒素.

Dinendra L Abeyawardhane, Spiridon E Sevdalis, Kaylin A Adipietro

    bioRxiv : the preprint server for biology
    |August 30, 2023
    PubMed
    概括

    Clostridioides 难毒素 (CDT) 使用 (Ca2+) 枯竭来触发宿主细胞中的孔隙形成. 这种机制与pH值变化不同,涉及与CDTbb分离.

    更多相关视频

    Deciphering the Molecular Mechanism and Function of Pore-Forming Toxins Using Leishmania major08:17

    Deciphering the Molecular Mechanism and Function of Pore-Forming Toxins Using Leishmania major

    Published on: October 28, 2022

    1.5K
    Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance10:41

    Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance

    Published on: January 3, 2012

    13.4K

    相关概念视频

    Clathrin Coated Vesicles01:12

    Clathrin Coated Vesicles

    Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
    7.1K
    Fusion of Secretory Vesicles with the Plasma Membrane01:26

    Fusion of Secretory Vesicles with the Plasma Membrane

    Proteins and neurotransmitters in secretory vesicles can be released from a cell upon vesicle docking, priming, and fusion with the plasma membrane. Vesicles are docked and primed in preparation for the quick exocytosis of their contents in response to a stimulus. The fusion process is mainly carried out by a SNAP Receptor or SNARE complex, consisting of synaptobrevin, syntaxin-1, and SNAP-25.
    In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
    11.1K
    Pinching-off of Coated Vesicles01:32

    Pinching-off of Coated Vesicles

    Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
    3.2K
    Mechanisms of Membrane Domain Formation00:59

    Mechanisms of Membrane Domain Formation

    Different physical properties of lipids and proteins allow them to localize and form distinct islands or domains in the membrane. Some membrane domains are formed due to protein-protein interactions, whereas others are formed due to the presence of specific lipids such as sphingolipids and sterols—for example, large proteins, such as bacteriorhodopsin, aggregate and create distinct domains.
    Another mechanism for membrane domain formation involves membrane proteins interacting with...
    3.0K
    SNAREs and Membrane Fusion01:43

    SNAREs and Membrane Fusion

    Once a transport vesicle has recognized its target organelle, the vesicular membrane needs to fuse with the target membrane to unload the cargo. Transmembrane proteins called SNAREs present on organelle membranes and their vesicles, mediate vesicle fusion.
    SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
    11.0K
    Protein Translocation Machinery on the ER Membrane01:28

    Protein Translocation Machinery on the ER Membrane

    The translocon complex situated on the ER membrane is the main gateway for the protein secretory pathway. It facilitates the transport of nascent peptides into the ER lumen and their insertion into the ER membrane.
    Sec61 protein conducting channel
    In eukaryotes, the translocon complex comprises a core heterotrimeric translocator channel called the Sec61 complex. This channel includes three transmembrane proteins, Sec61α, Sec61β, and Sec61γ, and is the largest subunit of the...
    4.7K