相关实验视频

Updated: Jul 17, 2025

High Resolution Fluorescent In Situ Hybridization in Drosophila Embryos and Tissues Using Tyramide Signal Amplification

High Resolution Fluorescent In Situ Hybridization in Drosophila Embryos and Tissues Using Tyramide Signal Amplification

Published on: October 19, 2017

用高多重复合RNA在人体组织上绘制地图 in situ 杂交化

Kian Kalhor^1,2, Chien-Ju Chen^1,3,2, Ho Suk Lee^1,4

¹Department of Bioengineering, University of California San Diego, La Jolla, CA, USA.

bioRxiv : the preprint server for biology

|August 30, 2023

概括

一种新的RNA in situ技术,DART-FISH,可以在大型人体组织中进行高分辨率的基因分析. 这种方法克服了以前技术的局限性,为生物医学研究和诊断提供了强大的工具.

科学领域:

分子生物学分子生物学
基因组学就是基因组学.
神经科学是一个神经科学.

背景情况:

多复合RNA在位杂交 (ISH) 技术为组织组织提供了洞察力.
现有的多重ISH方法在人体活检中面临局限性,原因是组织大小,质量和自身光.

结论:

DART-FISH是一种通用技术,用于大型人体组织的转录基因分析.
该方法增强了细胞分辨率,克服了临床活检分析中常见的挑战.
能够发现新的生物标志物,并对健康和疾病中的细胞状态进行表征.

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Visualizing Genetic Variants, Short Targets, and Point Mutations in the Morphological Tissue Context with an RNA In Situ Hybridization Assay

Visualizing Genetic Variants, Short Targets, and Point Mutations in the Morphological Tissue Context with an RNA In Situ Hybridization Assay

Published on: August 14, 2018

Whole Mount RNA Fluorescent in situ Hybridization of Drosophila Embryos

Whole Mount RNA Fluorescent in situ Hybridization of Drosophila Embryos

Published on: January 30, 2013

相关概念视频

In-situ Hybridization

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...