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Updated: Jul 17, 2025

GST-His purification: A Two-step Affinity Purification Protocol Yielding Full-length Purified Proteins
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使用GST融合系统在 prokaryotic 细胞中表达,净化和功能性 Teduglutide 的特征

Ali Akbar Alizadeh1, Saba Rasouli2,3, Omid Jamshidi Kandjani1

  • 1Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

Advanced pharmaceutical bulletin
|August 30, 2023
PubMed
概括

这项研究介绍了一种重组DNA方法,用于生产特杜格卢提德,用于治疗短肠综合征 (SBS). 该方法成功地表达,净化并证实了teduglutide的功能和结构,用于潜在的大规模制造.

关键词:
亲和色谱法是一种亲和色谱法.这是一种类.再组合技术是一种重组技术.在SBS中,SBS是SBS.尺寸排除色谱学 尺寸排除色谱学泰杜格卢提德 (Teduglutide) 是一种类型的类药物.

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Tagging and Fusion Proteins01:24

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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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