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Updated: Jul 17, 2025

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机器学习解决方案用于分析单粒子扩散轨迹.

Henrik Seckler1, Janusz Szwabiński2, Ralf Metzler1,3

  • 1Institute of Physics & Astronomy, University of Potsdam, 14476 Potsdam-Golm, Germany.

The journal of physical chemistry letters
|August 30, 2023
PubMed
概括

机器学习方法正在彻底改变分散运动数据的分析. 这一观点强调了用于时间序列分析的新机器学习方法,重点关注可解释性和不确定性估计.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
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