在新热带棕色臭虫Euschistus heros的转录组中转移的元素的注释及其染色体分布
Jaqueline Fernanda Dionisio1, Larissa Forim Pezenti1,2, Rogério Fernandes de Souza2
1Laboratório de Citogenética e Entomologia Molecular, Departamento de Biologia Geral, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, PR 445 Km 350, Campus Universitário, Caixa Postal: 10.011, Londrina, PR, CEP:86.057-970, Brazil.
Molecular genetics and genomics : MGG
|August 30, 2023
概括
这项研究在Euschistus heros转录组中确定了可移植元素 (TE),揭示了它们的染色体分布. 这些发现有助于更好地了解这一关键大豆害虫中TE动态.
科学领域:
- 基因组学就是基因组学.
- 分子进化分子进化
- 昆虫生物学 昆虫生物学
背景情况:
- 可转移元素 (TE) 是具有动态基因组分布的移动DNA序列.
- 了解像大豆主要害虫Euschistus heros这样的昆虫的TE多样性和演变仍然不完整.
研究的目的:
- 在E. heros转录组中识别可转换的元素组.
- 调查这些已识别的TE的染色体分布.
主要方法:
- 通过RNA-Seq和de novo组装生成了60009个转录.
- 布拉斯顿注释确定了367个可转移元素 (TEs).
- 光现场杂交 (FISH) 将选定的TE映射到染色体上.
主要成果:
- 第二类TE (DNA转体) 主要存在 (202/367),特别是TIR订单元素.
- 线性元素是最常见的I类TE (逆转移体).
- FISH发现了DNA转体子 (hAT,Helitron,Tc1-mariner) 和逆转体子 (Copia,Gypsy,Jockey,RTE) 的分散和阻断模式.
结论:
- 这项研究提供了第一个全面的识别和染色体映射TEs在E. heros.
- 整合基因组学和细胞遗传学对于理解昆虫基因组中的活性移动元素行为至关重要.
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